Abstract:Aim Mitofusion 2 （Mfn2） is a gene which can negatively regulate the hyperplasia of vascular smooth muscle cells （VSMC）. The gene is down-regulated in the VSMC of essential hypertensive patients. However,the potential mechanism is undiscovered. The object of this study is to investigate the mechanism of Mfn2 gene transcription and regulation by bioinformatics. Method By using online software,the promoter distribution,transcription factors and their binding sites,CpG island,TATA box,and conservative region were analyzed in the 5′ uncoding region of Mfn2 gene. Simultaneously,the distribution and function change of SNP were studied. Results There were five promoters in the 5′ uncoding region of Mfn2 gene,which two of them played a key role in the transcription of Mfn2. Sixty nine transcription factors′ binding sites were found in the two conservative districts. These transcription factors were mainly associated with inflammation,oxidative stress,hyperplasia and differentiation of cells,MAPK and STAT signal pathway. One CpG island was found. Eight of 15 TATA boxes were found in the region of －2000 bp upstream of the transcription origin site. Thirty two SNP sites were found,nine were in the core promoter region,and five may affect the transcription of Mfn2. Conclusion The core promoter distributes in the －400 bp region of 5′ uncoding region of Mfn2. There were a lot of transcription factors which were closely related with the function of Mfn2 existed. In addition,some SNPs which may affect the transcription regulation of Mfn2 were found in the promoter region.

Abstract:Aim To investigate whether bone marrow mesenchymal stem cells （MSC） reduce the apoptosis of human umbilical vein endothelial cells （HUVEC） induced by oxidized low density lipoprotein （ox-LDL）,and related mechanisms. Methods The HUVEC were divided into three groups: HUVEC were cultured in normol medium HUVEC were cultured with 100 mg/L ox-LDL for 24 h and HUVEC were cocultured with MSC with 100 mg/L ox-LDL for 24 h. Then cell apoptosis of different groups were mesured by flow cytometry. The content of VEGF,as well as TNF-α,in supernatant medium were determined by ELISA,and Real-time PCR was used to detect Bcl-2 and Bax mRNA expression. Results After 24 h stimulated by ox-LDL,the HUVEC apoptosis rate was increased,and the content of VEGF,TNF-α were significantly increased,while Bcl-2 mRNA expression was downregulated and Bax mRNA upregulated. The MSC cocultured with HUVEC could increase the VEGF levels,reduce TNF-α levels,as well as increase Bcl-2 mRNA expression but reduce the expression of Bax mRNA,HUVEC apoptosis was significantly decreased. Conclusions MSC can attenuate HUVEC apoptosis induced by ox-LDL,possibly through the increase of VEGF,reduction of TNF-α,and upregulation of Bcl-2 mRNA and downregulation of Bax mRNA,which provides a theoretical basis for MSC as a treatment for endothelial injury disease such as atherosclerosis.

Abstract:Aim To investigate the function of Sinapis alba extract’s antioxidant ability and regulation of CD36 expression in macrophages,and Sinapis alba extract’s prevention of atherosclerosis. Methods C57/BL6 mice were divided into two groups which were injected with 1.5% normal saline or 1.5% Sinapis alba extract for 0.2 mL/day,respectively. The injection was paused for one day after continuously injected for three days,and then on the 5^th day,mice were sacrificed to collect abdominal macrophages. Plasma superoxide dismutase （SOD） activity and lipid peroxide （LPO） cellular SOD,respiratory burst,LPO,expression of CD36 and neutral fat accumulation were assayed. 30 male ApoE^－/－ mouse were randomly divided into three groups: chew food diet group,high-fat diet group and high-fat diet ＋ 7.5% Sinapis alba group. The mouse were sacrificed 25 weeks later,and aorta was dissected,stained by oil red O. Plaque area was measured by an automatic image analyzer. Results In Sinapis alba extract group,SOD activity was higher and LPO was lower than that in the control group. Sinapis alba extract could reduce macrophage respiratory burst,and LPO level,CD36 expression and neutral fat accumulation induced by ox-LDL. Sinapis alba extract could reduce lipid plaque area by high-fat diet （P<0.05）. Conclusions Sinapis alba extract can effectively improve the body’s antioxidant capacity,improve the antioxidant activity of macrophages,reduce lipid uptake,prevent early atherosclerosis lesions.

Abstract:Aim To investigate the mechanism of endothelial nitric oxide synthase （eNOS） promoter DNA methylation in the damage of endothelial cells induced by homocysteine （Hcy）. Methods Human umbilical vein endothelial cells （HUVEC） were cultured with Hcy at different concentrations （0,50,100,200 and 500 μmol/L） and 100 μmol/L Hcy+Vitamin B12+folate respectively for 72 hours. The cell viability of HUVEC was detected by MTT,the mRNA expression of eNOS was analyzed by real-time PCR. The activity of eNOS was analyzed by ELISA,and the production of nitric oxide （NO） was examined by a commercial kit. Nested methylation specific polymerase chain reaction （nMS-PCR） was used for analysis of the methylation pattern in promoter regions of eNOS gene. The DNA methyltransferase activity was measured by isotope method. Results The viability of HUVEC exposed to different concentrations of Hcy decreased significantly. The mRNA levels of eNOS,eNOS activity and the NO production were significantly decreased. The methylation levels of eNOS promoter were Hcy-dose dependently increased,and the DNA methyltransferase activity was correlative increased. Conclusion Homocysteine can induce DNA hypermethylation of eNOS promoter,and the eNOS gene expression displayed correlative decrease,which may be an important mechanism for homocysteine-mediated endothelial impairment.

Abstract:Aim To regulate the expression of matrix metalloproteinase inducer （CD147） from Jurkat T cells,and observe its influence on monocyte chemotaxis. Methods Jurkat T cells were stimulated with cyclophilin A （CypA） of different concentrations,and the expression of CD147 protein from Jurkat T cells was determined with Western blot. Jurkat T cells were transfected with CD147-targeting small interfering RNA （siRNA） through the carrier Lipofectamine^TM2000 for 48 hours. The expression variation of CD147 mRNA and protein were determined by RT-PCR and Western blot respectively. The expression of CD147 from Jurkat T cells was up-regulated with CypA and down-regulated with CD147-targeting siRNA,then these Jurkat T cells and THP-1 monocytes were co-cultured in transwell assay to count the number of migrated THP-1 cells. Results Compared with blank control group,the expression of CD147 protein from Jurkat T cells increased along with the concentration gradient of CypA in the range of 0 to 400 μg/L （P<0.05）. Compared with the 400 μg/L CypA group,the expression of CD147 protein was not different with that of the 800 μg/L Cy-pA group （P＞0.05）. Compared with negative control siRNA,CD147 mRNA and protein levels decreased in Jurkat T cells transfected with CD147-targeting siRNA,and the inhibition ratios were 76.27%±2.00% and 71.67%±4.70% （P<0.05）. In the coculture system of Jurkat T cells pretreated with CypA and THP-1 cells,the number of migrated THP-1 cells （196.00±9.88 Cells/Field） significantly increased,compared with blank control group （109.00±8.22 Cells/Field P<0.05） however,in the coculture system of Jurkat T cells pretreated with siRNA and THP-1 cells,the number of migrated monocytes （44.00±6.98 Cells/Field） significantly decreased,compared with blank control group （109.00±8.22 Cells/Field P<0.05）. Conclusions CD147 expressed by Jurkat T cells was able to promote the chemotaxis of monocytes in coculture system.

Abstract:Aim To study the influence of plasma advanced oxidation protein products （AOPPs） level on the contents of high density lipoprotein （HDL） subclasses. Methods Contents of AOPPs were detected by the chloramine-T method and the composition of HDL subclasses were measured with two-dimensional electrophoresis immunoblot in 346 subjects. According to the concentration of AOPPs,subjects were divided into three layers amount: low AOPPs （AOPPs≤60 μmol/L） group,middle AOPPs （60 μmol/L＜AOPPs＜90 μmol/L） group,high AOPPs （AOPPs≥90 μmol/L） group. Results Compared with the low AOPPs group,preβ₁-HDL and HDL_3a were significantly increased （P<0.001）,but lecithin-cholesterol acyltransferase （LCAT）,HDL_2a and HDL_2b were significantly decreased （P<0.001） in high AOPPs group. Cholesterol ester transfer protein （CETP） did not change in subjects. Correlation analysis showed AOPPs effect on changes in plasma HDL subclass. Conclusion With elevated plasma AOPPs concentration,the particle size of HDL shows a shift toward smaller size,AOPPs may hinder mature metabolism of HDL.

Abstract:Aim To develop novel strategies to treat familial hypercholesterolemia （FH）,we explored knocking down the endogenous hydroxymethylglutaryl CoA reductase （HMG-CoAR） expression in C57BL/6J mouse by lentivirus mediated RNAi approach in this study. Methods Three lentiviral RNAi vectors targeting sites （T1,T2 and T3） in HMG-CoA reductase gene were subject to infect H22 cell line and tail vein injection to C57BL /6J mouse after viral packaging. HMG-CoAR mRNA abundance was measured by qRT-PCR Western Blot and ELISA assay were used to detect the HMG-CoAR protein expression in infected mouse liver or plasma. Results The expression of HMG-CoAR in stably transfected H22 cells of three experimental groups was dramatically reduced in comparison to control （P<0.05）. Significantly lowing HMG-CoAR was detected in mouse liver and plasma 5 days after infection （P<0.05）. Among the three target sites,T3 was proved to be the most effective,in which the HMG-CoAR level was only 1/3 of control group. Moreover,the result of time course analysis of the inhibition effects showed that the HMG-CoAR expression was supressed at least up to 50 days in all three groups. T1 site exhibited most stable inhibition effect,yet T3 site showed more fluctuating though it was more effective than others. Conclusion Three RNAi targeted vectors were demonstrated to be able to inhibit endogenous HMG-CoAR expression in cell line and mouse. The results pave the way for lowing serum lipid in FH treatment by knocking down HMG-CoAR level.

Abstract:Aim To study the effects of Echinocystic acid on the protein expression of apoptosis-associated Bcl-2,Bax and PARP （89 kDa） in the primary cultured rat cardiomyocytes subjected to anoxia/reoxygenation injury. Methods The cardiomyocytes were divided into normal group,anoxia/reoxygenation injury group,anoxia/reoxygenation preconditioning group,different concentration Echinocystic acid groups （0.5 μmol/L,5 μmol/L and 50 μmol/L）. Cardiomyocytes were subjected to anoxia for 3 hours and subsequently reoxygenation for 2 hours. Cell viability and LDH activity in medium were measured. The expression o f Bcl-2 and the apoptotic protein Bax and PARP （89 kDa） were detected by Western Blot. Results Compared with that of the control group,the expression of Bcl-2 protein in cardiomyocytes decreased significantly （P<0.01） and the expression of Bax protein and PARP （89 kDa） in cardiomyocytes increased significantly （P<0.01） after reoxygenation. Cell viability decreased obviously （P<0.05）. Compared with that of the anoxia/reoxygenation group,pretreatment with different concentration Echinocystic acid decreased LDH activity and increased cell viability（P<0.05）,the expression of Bcl-2 protein in the Echinocystic acid （5 μmol/L） groups increased significantly （P<0.05） and the expression of Bax protein decreased significantly （P<0.05）. Conclusion The antiapopotic effects of Echinocystic acid might be attributed to the up-regulated expression of Bcl-2 gene and the inhibited expression of Bax and PARP（89 kDa） gene expression.

Abstract:Aim To observe the role of Ghrelin on myocardial metabolism key enzyme AMP-activated protein kinase （AMPK） signaling pathway. Methods Goto-Kakizaki （GK） rats （8-week-old males） were randomly divided into three groups with 8 rats in each group: GK group,metformin group（received metformin 350 mg/kg）,and Ghrelin group （received Ghrelin 200 μg/kg in twice daily）. Normal male Wistar kyoto rats （n8） served as normal controls. Four weeks later,the effects of Ghrelin on cardiac remodeling were evaluated by echocardiographic,hemody- namicand gene expression analysis （AMPK,Glut4 mRNA expression）. Results After 4 weeks,compared with Wistar rats and GK group,heart rate reduced,diastolic,systolic function improved in Ghrelin group （P<0.05） HOMA-IR index was lower than the GK rats in Ghrelin group （P<0.05） Myocardial mitochondria and myocardial microvascular of Ghrelin group rats were close to Wistar group. Myocardial AMPKα₂,Glut4 mRNA expression levels were significantly increased in Ghrelin group compared with GK group （P<0.05）. Conclusions Intraperitoneal injection of Ghrelin can reduce the spontaneous GK rats insulin resistance,and improve left ventricular diastolic and systolic function. Its mechanism may be associated with longer-term increase of AMPK expression of myocardial cell and the promotion of the glucose transporter.

Abstract:Aim To explore the possible mechanism by which probucol influenced cellular cholesterol efflux,and observe effects of serum from the mice pretreated by probucol on cellular cholesterol efflux. Methods Male C57BL/6J mice （n16） were randomly divided into two groups and treated with either vehicle or 0.5% probucol for 4 weeks respectively,the serum was collected and the lipids profile was detected by enzymatic method. Macrophages were loaded with ac-LDL and labeled with ³H-cholesterol,and treated with different concentrations of probucol,then the efflux of cholesterol was quantitated using the serum mentioned above as the acceptors. mRNA and membrane protein of the treated cells were extracted and used to evaluate the mRNA and protein expression of ABCA1,ABCG1 and SR-BI. Results More cholesterol efflux from the cultured macrophages was mediated by the serum from the mice treated with probucol for 4 weeks than those from the control mice. Probucol dose-dependently promoted cholesterol efflux and up-regulated the mRNA and protein expression of ABCG1 in macrophages,while had no effect on the mRNA and protein expression of SR-BI and ABCA1. Conclusions The serum from the mice pretreated with probucol markedly promoted macrophages cholesterol efflux. Probucol maybe promoted cholesterol efflux from the macrophages through increasing the expression of ABCG1 but had no relation to the expression of ABCA1 and SR-BI.

Abstract:Aim To investigate the effects of nicotinic acid on the atherosclerotic plaques formation and osteopontin （OPN） mRNA expression of aorta. Methods 16 New Zealand white rabbits were fed with high-fat diet for 8 weeks,and then were randomly divided into two groups: （1）hyperlipidemia group （n＝8）: maintained high-fat diet for 6 weeks （2）niacin treatment group （n＝8）: the same fat diet supplemented with niacin ［200 mg/（kg·d）］ for 6 weeks. Control group （n＝8） was fed with normal diet for 14 weeks. At the end of the study,all rabbits were sacrificed to detect the histopathological changes and OPN mRNA expression of the aortas. Results Rabbits fed with high-fat diet showed more aorta endometrial thickness,plaque area and OPN mRNA expression （all P<0.01）. Compared with hyperlipidemia group,rabbits treated with niacin showed less aorta endometrial thickness,plaque area and OPN expression （all P<0.01）. Correlation analysis suggested that expressions of OPN mRNA （r＝0.888,P<0.01） and OPN protein （r＝0.821,P<0.01） were positively correlated with area of atherosclerotic plaque,expressions of OPN mRNA （r＝0.874,P<0.01） and OPN protein （r＝0.818,P<0.01） were also positively correlated with intimal thickness of aorta. Conclusion Niacin’s role of anti-atherosclerosis not only is due to its lipid-lowering effect,but also may be related to its OPN expression-lowering effect.

Abstract:Aim To explore the effect of acid fibroblast growth factor （aFGF） on the apoptosis in endothelial progenitor cells （EPCs） from human umbilical cord blood （HUCB）. Methods Mononuclearcells （MNCs） were isolated from HUCB in vitro by Ficoll density gradient centrifugation,then the cells were plated on fibronectin-coated culture dishes. Aftert 7 days,the attached cells were treated by aFGF with different concentrations （2,5,10 μg/L） for 24 hours. EPCs were characterized as adherent cells with double positive to DiI-acetylated low density lipoprotein （DiI-acLDL） uptake and lectin binding by direct fluorescent staining under a laser scanning confocal microscope. Flow cytometry was used to detect cell apoptpsis. The expressions of Bcl-2 mRNA and protein were detected respectively by reverse transcription polymerase chain reaction （RT-PCR） and Western Blot. Meanwhile,the attached cells in the group （5 μg/L aFGF group） of the most obvious effects on EPCs were cultured for 6,12,24,48 h respectively,accordingly,to explore the relationship between time and effect of 5 μg/L aFGF group. Results Compared with control group,aFGF can argument the number of EPCs and inhibit apoptosis of EPCs （P<0.05）. The increase and inhibition ratio of apoptosis reached the maximum at 24 h after administration of 5 μg/L aFGF （P<0.05）. Expression of Bcl-2 mRNA and protein of EPCs in aFGF group was higher than that in control group （P<0.05）. Conclusions The results of the present study define a novel mechanism of the action of aFGF aFGF can augment the number and inhibit apoptosis of EPCs from HUCB via up -regulating Bcl-2 expression.

Abstract:Aim To know means and distribution of cholesterol synthesis and absorption markers in healthy persons. Methods 64 healthy adults were selected in medical examination center of Anzhen hospital and conventional determination of liver and kidney function,serum total cholesterol （TC）,high density lipoprotein cholesterol （HDLC）,triglyceride （TG）,fasting plasma glucose （FPG）. Serum markers of cholesterol synthesis and absorption were determined with gas chromatogram. Results The sum of the average of five kinds of non-cholesterol steroids was 1.02 mg/dL,approximately 0.72% of total cholesterol,and there were individual differences. Serum lathosterol level in male was significantly higher than women （P<0.05）,while serum stigmasterol in male were significantly lower than women （P<0.01）. Synthesis markers of squalene decreased with the age increasing and absorption markers of stigmasterol increased with the age increasing. Conclusions It is through the detection of cholesterol absorption and synthesis markers to learn the distribution of cholesterol metabolism markers in healthy individuals.

Abstract:Aim To analyze correlation between the N-terminal brain natriuretic peptide precursor （NT-proBNP） levels and severity of coronary artery disease in patients with acute coronary syndrome （ACS）. Methods 40 patients was diagnosed with ACS combined coronary angiography and history,replacement of the 20 cases in the control group was coronary angiography negative. Gensini score was used for the evaluation of coronary lesions severity. According to Gensini score,ACS patients were divided into the Gensini score ≥30 group and <30 group. And according to the number of diseased vessels,ACS patients were divided into single,double and three vessels disease groups. Immunoassay analyzer and ancillary reagents were used for determination of plasma NT-proBNP level in all patients. The correlation between NT-proBNP levels and the severity of coronary artery disease was analyzed. Results NT-proBNP levels of ACS group were significantly higher than that in the control group ［242.56 ± 68.16 ng/L vs 27.12 ± 16.48 ng/L,P<0.01］. The level of NT-proBNP in three-vessel disease group was higher than in single-vessel disease group （P<0.05）. In Gensini score ≥30 group,NT-proBNP level was significantly higher than that in Gensini score <30 group ［228.14 ± 46.16 ng/L vs 127.64 ± 32.64 ng/L,P<0.05］. Plasma NT-proBNP level was positively correlated with the Gensini score （r0.56,P<0.05）. Conclusions Plasma NT-proBNP levels in patients with ACS has clinical significance in the evaluation of coronary artery disease severity.

Abstract:Aim To determine the correlation of the carotid-femoral pulse wave velocity （cfPWV） with plasma high molecular weight-adiponectin （HMW-ADP） level of dewelling persons. Methods 87 dewelling persons who underwent health examination in medical examination center were surveyed in this experiment. The clinical data of subjects contained age,gender,waistline,systolic blood pressure （SBP）,diastolic blood pressure （DBP）,hepatic function,renal function,high density lipoprotein cholesterol （HDLC）,low density lipoprotein cholesterol （LDLC）,triglyceride （TG）,total cholesterol （TC）,fasting blood glucose （FBG）,postprandial 2 hour plasma glucose （2hPG）,fasting C-peptide,plasma total adiponectin level,plasma HMW-ADP level,cfPWV,HOMA-IR,and body mass index （BMI）. All subjects were divided into two groups in which group A contained 21 cases （cfPWV < 9 m/s） and group B contained 66 cases （cfPWV≥9 m/s）. Results The difference of BMI,SBP,DBP,the serum levels of HDLC,LDLC,TG,TC,total-ADP,and HMW-ADP between the two groups had statistical significance （P<0.05）,however,no significant differences occurred in age,gender,total protein,globulin,albumin,total bilirubin（TBIL）,conjugated bilirubin（CB）,aspartate aminotransferase（AST）,alanine aminotransferase（ALT）,blood urea nitrogen（BUN）,serum creatinine （Scr）,serum uric acid（UA）,fasting blood glucose （FBG）,postprandial 2 hour plasma glucose（2hPG）,fasting C-peptide,fastinginsulin（FIN） and homeostasis model assessment of insulin resistance （HOMA-IR）（ P>0.05）. Multivariate regression analysis revealed that the serum levels of HMW-ADP,total-ADP,and HDLC were negatively correlated with cfPWV （OR＾0.011,0.019,0.125P0.011,0.012,0.015）,while SBP was positively correlated with cfPWV（OR＾1.046,P0.045）. Conclusions High levels of HMW-ADP and total-ADP in serum may be the protective factors for angiosclerosis.

Abstract:Aim To investigate whether increased plasma levels of serum 25-hydroxy vitamin D ［25（OH）VD］ are associated with cardiac structural and functional abnormalities in obstructive sleep apnea-hypopnea syndrome （OSAHS） patients. Methods We documented 337 consecutive patients with OSAHS. Left ventricular hypertrophy （LVH） ［left ventricular mass index （LVMI）≥125 g/m² in male,and LVMI≥120 g/m² in female］ was determined by echocardiography. The serum 25-hydroxy vitamin D concentration was measured by using enzyme-linked immunosorbent assay （ELISA） method. Results The LVMI,prevalence rate of LVH and body mass index （BMI） were higher in the subjects with severe OSAHS （apnoea-hypopnoea index≥30/h,LVH 75%,n＝226） than in those with mild to moderate OSAHS （LVH 40%,n＝111）（P<0.01）,and serum 25（OH）VD levels were lower in the severe OSAHS groups. Although the serum 25（OH）VD level showed a negative correlation with BMI,the results of binary logistic regression analysis demonstrated that the quartile value of 25（OH）VD was an independent significant variable for the identification of LVH （adjusted OR in quartile＝4.11,95%CI: 1.15-12.70,P<0.01）,even after adjusting for obesity and other risk factors. Conclusion Decreased serum levels of 25（OH）VD do seem to reflect an increased likelihood of LVH in patients with severe OSAHS.

Abstract:Aim To investigate the prevalence and risk factors of hypertension among unban residents,and to provide the scientific evidence for the intervention measurements. Methods The residents,more than 35-year-old,were selected based on a cluster sampling design. Questionnaire survey and medical examination were used. The data were delivered by Epidata 3.1 and analyzed by SPSS 13.0. Results In all the residents studied,the incidence of hypertension was 46.5%. The incidence between male（51.9%） and female（41.3%） had a significant difference （P<0.0001）,and the prevalence of hypertension increased with age. The rates of hypertension awareness and control of this community were 70.2%and 23.8%. Compared with those with normal body mass index （BMI）,the prevalence of hypertension in patients with overweight and obesity were significantly increased. The prevalence of hypertension in patients with impaired fasting glucose and diabetes mellitus were significantly higher than those with normal glucose tolerance（51.5%vs 36.1%,59.0%vs 36.1%,respectively,P<0.0001）. Stepwise Logistic regression revealed that age，male，drinking,overweight/obesity，glycometabolism abnormality and high triglycerides level were independent risk factors of hypertension （P<005）. Conclusion The incidence of hypertension in Hangzhou district in Zhejiang province is relatively high. The distribution and relevant risk factors have its local characteristics. Thus,the hypertension control and comprehensive preventive measures should be implemented.

Abstract:Under conditions of hypoxia generalized vasoconstriction and remodeling of the pulmonary vascular leads to pulmonary vascular persistent resistance. The medial thickening is the main reason of pulmonary vascular remodeling and hypoxic pulmonary artery hypertension，pulmonary artery smooth muscle cells （PASMC） are the principal structure of media,and chronic hypoxia induces the imbalance of vasoactive substances and growth factors. Under this condition,the main medial thickening is believed to be attributable to proliferation,hypertrophy and increased accumulation of PASMC as well as expression of extracellular matrix proteins. Moreover,PASMC has an interaction with endothelial cell of intima and fibroblast of adventitia through multiple signal pathways and plays a crucial role in the development of pulmonary vascular remodeling. The article will make a summary of latest research on PASMC and mechanism of hypoxic pulmonary vascular remodeling.

Abstract:Hydrogen sulfide is a toxic gas with rotten eggs taste. However,there were a large number of articles reported that hydrogen sulfide was the third gaseous signaling molecule after CO and NO in recent years. Hydrogen sulfide derived mainly from cystathionine b-synthase,cystathionine c-lyase and 3-Mercaptopyruvate sulfurtransferase enzyme,which had many roles such as blood vessel dilatation,regulating blood pressure,inhibiting vascular smooth muscle cell proliferation and low density lipoprotein （LDL） oxidative modification. Especially hydrogen sulfide has a significant protective role on ischemic myocardial cells. Studies have shown that the potassium channels may be involved in various forms and multiple organ ischemia protection. Hydrogen sulfide is an important gaseous signaling molecule,of which improving blood perfusion levels may be related to the potassium channel. This review focuses on the role of potassium channel during the process of hydrogen sulfide producing biological effect.

Abstract:Diabetes mellitus is a risk equivalent of coronary heart disease and the pathogenesis of diabetic coronary atherosclerosis （As） is complicated,long-term hyperglycemia is recognized as the primary culprit for it can damage the endothelial cells persistently through interfering the intracellular signaling pathway and metabolism. Researches show that the increase of chronic inflammation and excessive oxidative stress is the most important pathophysiologic mechanisms for As and the common pathway of pathogenesis under the circumstance of hyperglycemia. In recent years,the study found that the hemoglobin scavenger receptor （CD163） has an obvious anti-inflammatory and anti-lipid peroxidation capacity and may play an important role in the prevention of diabetic coronary As.