Abstract:Aim To assess the effects of vitamin K on calcification and the expression of Toll-like receptor 2 （TLR2） as well as Toll-like receptor 4（TLR4） in aortic artery of ApoE^－/－ mice. Methods Eighteen 6-week-old male ApoE^－/－ mice were randomly divided into model group,interventional group and control group. Model group and interventional group were fed with high-fat diet,while control group were fed with normal diet. Mice in interventional group were administered orally with vitamin K2,once a day for 12 weeks. All mice were sacrificed when the mice were 19 weeks old,blood was collected and plasma triglyceride （TG）,total cholesterol （TC）,high density lipoprotein cholesterol （HDLC）,low density lipoprotein cholesterol （LDLC） were measured. Aortic sections were stained with hematoxylin and eosin （HE） or Von Kossa,and observed under microscope. Calcium content and alkaline phosphatase activity of aorta were measured to determine the degree of vascular calcification. The expressions of TLR2 and TLR4 protein and mRNA levels in aotic sections of mice were detected by immunohistochemisty and qRT-PCR respectively. Results HE staining demonstrated significant thickening of the intima,with typical atherosclerotic plaque formation in model group mice,and von Kossa staining showed spotty black clumps of aortic calcification under the fibrous cap plaque,while intervention group had atherosclerotic plaques without the formation of significant calcium deposits The quantitative analysis showed that aortic vascular wall calcium and alkaline phosphatase activity were significantly lower in the intervention group than that of the model group （P<0.01） Immunohistochemical staining showed that TLR2,TLR4 mainly expressed in atherosclerotic plaques,qRT-PCR confirmed that the expression of TLR2 and TLR4 was significantly higher in the model group mice aorta （P<0.05）,while the expression level of TLR2 and TLR4 mRNA or protein were significantly lower in intervention group aortic than in the model group. The expression level of TLR2 and TLR4 mRNA in mice aorta tissue was positively related to the content of calcium（r0.77 and r0.79,respectively,both P<0.001）. Conclusions Vitamin K2 can reduce calcium content and alkaline phosphatase activity,and inhibit intimal calcification induced by high-fat diet in ApoE^－/－ mice. The results suggest that the effects of vitamin K2 on calcification of aortic artery may be associated with the regulation for the expression of TLR2 and TLR4.

Abstract:Aim To investigate the effect of β-nerve growth factor （β-NGF） gene transfected human umbilical vein endothelial cells （HUVEC） on cell function related to proliferation,monoclonal formation,cell cycle,apoptosis,migration. Methods The recombination human β-NGF gene expression eukaryotic plasmid with EGFP （pEGFP-N1） was transfected into HUVEC,HUVEC without transfected plasmid as the normal control group,HUVEC with empty vector （pEGFP-N1） as the negative control group,and-NGF gene transfection with the 1∶1000 K252a as the tyrosine receptor kinase A （TrkA） inhibition group.

Abstract:Aim To investigate the antagonistic effect of amlodipine on oxidized low density lipoprotein （ox-LDL） induced rat marrow endothelial progenitor cells （EPC）vascular tube-like information injury and related mechanisms.

Abstract:Aim To explore the hyperhomocysteinemia （HHcy） inhibit cystic fibrosis transmembrane conductance regulator （CFTR） expression of apolipoprotein E-knocked-out （ApoE^－/－） mice renal via endoplasmic reticulum stress （ERS）. Methods 18 male ApoE^－/－ mice were randomly divided into 3 groups （n＝6 for each group）: ApoE^－/－control group,high methionine group and intervention group. They were respectively fed with nomal diet and high methionine diet and high methionine diet plus 0.006% folate and 0.0004% vitamin B₁₂ for 14 weeks. After 14 weeks,the blood of the mice was gotten using a unilateral enucleation method,the serum homocysteine （Hcy） was detected by enzyme-linked immunosorbent assay （ELISA）. The protein and mRNA expressions of CFTR in the renal of mice were detected by immunohistochemical assay and real time polymerase chain reaction （RT-PCR） the mRNA expressions of glucose regulated protein 78 （GRP78）,activating transcription factor 6 （ATF6）,protein kinase RNA-like endoplasmic reticulum kinase （PERK）,C/EPB homologous protein （CHOP） in the renal of mice were detected by RT-PCR. Results The result showed that the content of Hcy was significantly higher in ApoE^－/－control group,high methionine group and intervention group an remarkable increase was observed in the high methionine group （P<0.01）. The results of RT-PCR and immunohistochemical assay exposed a remarkable down-expressed CFTR mRNA and protein in high methionine group （P<0.01）. The results of RT-PCR exposed a remarkable up-expressed GRP78,ATF6,PERK,CHOP mRNA in high methionine group,while intervention group decreased GRP78,ATF6,PERK,CHOP levels compared with high methionine group. There were negative correlations between GRP78,ATF6,PERK,CHOP mRNA and CFTR mRNA （r＝－0.7192,P<0.01 r＝－0.5501,P<0.01 r＝－0.7772,P<0.01 r＝－0.6785,P<0.01）. Conclusion HHcy inhibits CFTR expression of ApoE-knockout mice renal might via endoplasmic reticulum stress.

Abstract:Aim To assess myocardial fibrosis with ultrasonic backscatter measurement combined with collagen metabolite in ischemia/reperfusion （I/R） injury canine. Methods 12 adult mongrel dogs were randomly divided into 2 groups: sham operation group （sham group） and ischemia/reperfusion group （I/R group）,6 dogs in each group. Integrated backscatter （IBS） and cyclic variation of integrated backscatter （CVIB） were detected in ventricular septal and posterior wall of the left ventricular with quantitative analysis of ultrasonic backscatter. Serum levels of connective tissue growth factor （CTGF） and aminoterminal propeptide of type Ⅰ procollagen （PⅠNP） were examined by enzyme-linked immunosorbent assay （ELISA）. Myocardial fibrosis of ventricular myocardium was tested by Masson staining,and expression of collagen Ⅰ was detected with pathologic immunohistochemistry. Results Compared with sham group,CTGF and PⅠNP levels were increased,IBS significantly was enlarged,and CVIB significantly was decreased in I/R group,the differences were statistically significant （P<0.05）. CTGF and PⅠNP levels were positively correlated with IBS and negatively correlated with CVIB in I/R group （P<0.05）. Masson staining showed that myocardial fibrosis was significantly increased and expression of collagen Ⅰ protein was strongly positive in I/R group. Conclusions IBS is increased,CVIB is decreased,and CTGF and PⅠNP levels were increased in ischemia/reperfusion injury canine. Myocardial fibrosis was involved in I/R damage to induce ventricular remodeling. Ultrasonic backscatter measurement combined with collagen metabolites could be an early and non-invasive method to examine ventricular fibrosis degree at I/R stage.

Abstract:Aim To investigate the effect of high saturated fatty acids and n-3 polyunsaturated fatty acid diet on arterial elasticity in spontaneously hypertensive rats （SHR）. Methods Thirty male SHR （8 weeks old）,adaptive feeding to 14 weeks old,were randomly divided into 3 groups by body mass: normal chow group（NFD）,high-fat diet group （HFD） and group of high-fat diet supplemented with fish oil group（HF+FOD） to 36 weeks old were intervened for 22 weeks. Thirty male Wistar-Kyoto rats（WKY） at the same age were divided into the same groups. After being intervened,the blood pressure,weight,serum free fatty acids （FFA）,biochemical index were measured. At the end of the feeding,artery pulse wave velocity （APWV）,using histochemical,pathological,change of centre arterial vascular wall and matrix metalloprotein-9 （MMP-9） were measured. Results Systolic blood pressure （SBP） in SHR+HFD group was higher than that in SHR+NFD group and SHR+HF+FOD group （160.5±5.5 mmHg vs 153.1±1.0 mmHg,155.1±6.7 mmHg,both P<0.01）. Compared with the SHR+NFD group,the membrane cross-sectional area （MCSA） increased （35500±8536 μm² vs 28348±3956 μm²,P<0.05）,aortic elastic properties in the SHR+HF group significantly decreased （21.1%±2.3% vs 30.7%±2.3%,P<0.05）. However,in SHR+HF+FOD group,such a marked increase of MCSA （16865±7235 μm² vs 35500±8536 μm²,P<0.05） and decrease of aortic elastic properties （34.5%±4.1% vs 21.1%±2.3%） were completely reversed （P<0.05）. The APWV was increased in the SHR+HF group compared with the SHR+NFD group. But it was significantly decreased in SHR+HF+FOD group compared with SHR+HF group. MMP-9 expression was higher in SHR+HFD group compared with that in SHR+NFD group and SHR+HF+FOD group. Conclusion The diet of high saturated fatty acid stimulates the thickness of aorta blood vessel in SHR and increases arterial stiffness,raises MMP-9 expression,dietary fish oil reduces MMP-9 expression,vascular wall thickness and increases aortic contents of elastin in SHR. This may contribute to decrease blood pressure.

Abstract:Aim To investigate the pathological changes of vascular remodeling and collagen in spontaneously hypertensive rats（SHR） and the effect of treatment by Xuezhikang. Methods 30 male SHR at 8-week-old were randomly divided into three groups （n10 each）: Xuezhikang low dose group （XZK-L group,Xuezhikang 20 mg/（kg·d））,Xuezhikang high dose group （XZK-H group,Xuezhikang 200 mg/（kg·d）） and placebo group,sex and age matched Wistar rats （control group,n10） were also designed. 8 weeks after intragastric administration,the levels of serum matrix metalloproteinase-9（MMP-9） and tissue inhibitors of metalloproteinase-1（TIMP-1） were measured by enzyme linked immunosorbent assay （ELISA）. The thoracic aortas were collected for hematoxylin-eosin （HE） stain to detect vascular wall thickness and Wall-to-lumen area ratio （W/L）. The aortic collagen was showed by Masson trichrome stain. The thoracic aortas were observed by immunohistochemistry to detect the expressions of MMP-9 and TIMP-1. Quantitation of the protein content of MMP-9 and TIMP-1 was assessed by computerized planimetry in the aortic media in immunohistochemically stained slides by using Image-Pro Plus 6.0. Results The level of serum MMP-9 in placebo group was significantly increased compared with XZK-H group and control group （P<0.05）. The levels of serum TIMP-1 in the SHR groups were higher than control group（P<0.01）. There was no significance of the levels of TIMP-1 in all SHR group （P>0.05）. Aortas of SHR showed wall thickening,increasing wall-to-lumen area ratio and collagen,disordered fibres. The thickness of vascular wall and W/L ratio of SHR were significantly higher than those of Wistar rats （P<0.05）. Arteries in XZK group showed less collagen in aortic wall than that of placebo group,especially the XZK-H group was near to the control group. In the placebo group,MMP-9 protein expression was significantly increased in the thoracic aorta compared with control group and XZK-H group（P<0.05）. However,the level of TIMP-1 had no significance in the four groups （P>0.05）. Conclusions Hypertensive vascular remodeling shows elevated level of MMP-9 and collagen deposition. While Xuezhikang can inhibit the elevated MMP-9 and collagen deposition,improve vascular remodeling in hypertension.

Abstract:Aim To explore the expression of micorRNA-503 and its target genes,in process of diabetes mellitus induced impairment reparative angiogenesis after acute myocardial infarction. Methods GK rats and Wistar rats 30 each were made into the model of acute myocardial infarction by ligating the left anterior descending coronary artery. Capillary density around the region of infarction was detected by immunohistochemical staining with vWF,expression of micorRNA-503 was measured by qRT-PCR,and the protein expressions of cyclinE1 and cell division cycle 25A （CDC25A） were measured by Western blot. Analysis of cappilaries,micorRNA-503,cyclinE1 and CDC25A were made on days 0（pre-ligation）,3,7,14 and 28 days post-ligation. Results In each time point post-ligation,the capillary density around the region of infarction and expression of CDC25A decreased in GK rats compared with Wistar rats,the expression of micorRNA-503 increased significantly （P<0.05）. Decreased expression of cyclinE1 in GK rats was only observed on 14 and 28 days post-ligation （P<0.05）. Conclusion Our data suggest that micorRNA-503 contribute to diabetes mellitus induced impairment reparative angiogenesis after acute myocardial infarction,via down-regulation of CDC25A.

Abstract:Aim To observe the effects of alprostadil on the expressions of metalloproteinase-2 （MMP-2） and metalloproteinase-9 （MMP-9） and fibrosis in myocardial tissues of rats with chronic heart failure. Methods Seventy male SD rats were randomly divided into sham operation group and model group. Rats in model group were made by abdominal aorta coarctation. Four weeks later,the model rats were randomly divided into four groups according to different concentrations of alprostadil: untreated group,low dose group,medium dose group and high dose group. After two weeks,myocardial collagen fibers were evaluated through Masson staining and collagen volume fraction （CVF） was measured. The contents of hydroxyproline （HYP） and the total collagen in myocardium were tested. The mRNA expressions of MMP-2 and MMP-9 in myocardial tissues were detected by real-time fluorescence quantitative polymerase chain reaction and activities of MMP were assayed by zemography. Results Compared with untreated group,CVF,HYP and the total collagen contents in myocardial tissues of rats were decreased significantly （P<0.05 or P<0.01） in all drug-treated groups. The mRNA expression levels and activites of MMP-2 and MMP-9 were significantly lower in drug-treated groups than in untreated group （P<0.05 or P<0.01）. There was no statistically signicant difference of the mRNA expression between low dose group and medium dose group （P>0.05）. The mRNA contents of MMP-2 and MMP-9 were decreased significantly in high dose group compared with low dose group and medium dose group （P<0.05）. The levels of MMP-2 and MMP-9 activates were significantly decreased with the concentration of alprostadil （P<0.05 or P<0.01）. Concentrations of alprostadil are inversely correlated with CVF,HYP,total collagen contents,mRNA expressions and activates of MMP-2 and MMP-9 in myocardial tissues of rats （P<0.05 or P<0.01）. Conclusions Alprostadil can decrease the expressions and activition of MMP-2 and MMP-9 in myocardial tissue of rats with chronic heart failure,which may be the important mechanism in delaying the myocardial fibrosis progression,preventing cardiac remodeling and delaying the progress of chronic heart failure.

Abstract:Aim To investigate the effect of Procyanidins on vascular endothelial cell in diabetic rats and discuss the possible mechanism. Methods Male SD rats were injected streptozotocin （ STZ， 30 mg/k g ） by tail intravenous for establishing the type I diabetes animal model，randomly divided into diabetes model group，50 mg/kg procyanidins treatment group and 100 mg/kg procyanidins treatment group，and other 8 healthy male SD rats were included into the control group. The rats took medicine by irrigation stomach for 12 weeks,rat fasting blood glucose was detected with glucose meter and special in-situ；serum ET-1 and PGI2 level were detected with radiation immunity analyzer；MDA and SOD in vascular endothelial cell were detected by the methods of ELISA Caspase-3 ,Bax and Bcl-2 in vascular endothelial cell were detected by the conventional immunohistochemical treatment and dyeing methods；vascular endothelial cell microscopic image was observed and collected with being future-proof imaging analysis system （× 400） at high magnification；Caspase-3 ,Bax and Bcl-2 in vascular endothelial cell were analysed by mean-density using the microscope Image analysis software Image - Pro Plus 6.0 in yellow. Results Compared with the normal control group,in Diabetes model rats,rat fasting blood glucose level increased， serum ET-1 level increased and PGI2 level reduced （P< 0.01）,MDA in vascular endothelial cell increased（P< 0.01）,SOD in vascular endothelial cell reduced（P< 0.01）,Caspase-3 and Bax in vascular endothelial cell increased（P< 0.01） Bcl-2 and Bcl-2/Bax in vascular endothelial cell reduced（P< 0.01）In 50 mg/kg procyanidins treatment group and 100 mg/kg procyanidins treatment group,rat fasting blood glucose level decreased （P< 0.05，P< 0.01），serum ET-1 level reduced and PGI2 level increased（P< 0.01）,MDA in vascular endothelial cell reduced （P< 0.01）,SOD in vascular endothelial cell increased （P< 0.05，P< 0.01）,Caspase-3 and Bax in vascular endothelial cell reduced （P< 0.01） Bcl-2 and Bcl-2/Bax in vascular endothelial cell increased （P< 0.01）. Conclusion Procyanidins has protective effect on diabetic rats vascular endothelial cell secretion function and oxidative damage,meanwhile can inhibit Caspase-3 and Bax ,induce Bcl-2 and Bcl-2/Bax of diabetic rat vascular endothelial cell.

Abstract:Aim To investigate relationship between the expression of microRNA （miRNA） and inflammatory cytokines in plasma in patients with acute cerebral infarction （ACI） and its clinical significance. Methods Sixty patients with ACI were divided into three groups according to clinical neurological deficit score. The patients were further divided into non-survival group and survival group based on their outcomes. 20 Healthy volunteers were enrolled into the control group. The expression levels of plasma miR-210,miR-145,miR-21,miR-223,miR-497,miR-15a were detected by real-time quantitative PCR（qRT-PCR）. Tumor necrosis factor-α （TNF-α） and interleukin-10 （IL-10） levels in plasma were detected by enzyme linked immunosorbent assay （ELISA）. Results Expression of miRNA and level of TNF-α,TNF-α/IL-10,IL-10 were significantly increased in patients with ACI compared with normal control group Expression of miRNA and level of TNF-α,TNF-α/IL-10,IL-10 were gradually increased. Among the patients,the level of TNF-α,TNF-α/IL-10,IL-10 in the survival group were obviously lower than those in the non-survival group （P<0.05 or P<0.01）. There was a positive correlation between miR-21,miR-223 and IL-10 and IL-10/TNF-α（ P<0.01）. Conclusion The expression levels of miR-21 and miR-223 in plasma in patients with ACI was significantly upregulated,and had a positive correlation with IL-10 and IL-10/TNF-α,which may be used as early diagnostic markers and can reflect the severity of condition to a certain degree.

Abstract:Aim To explore the prevalence of the cluster of cardiovascular risk factors in the races of the Uygur and the Hans in hospital with insulin resistance（IR） in Urumqi. Methods Oral glucose tolerance test （OGTT） and insulin release test were used to measure concentration of blood glucose and insulin before and after oral 75 g glucose in 0 h,0.5 h,1 h,2 h and 3 h,which were recorded in 219 subjects in hospital. Insulin resistance （HOMA IR） were calculated by Homeostasis model assessment （the Homa model）. Results In the insulin resistance subjects,the Uygur increased significantly in weight and waist-to-hip ratio compared with the controls,whereas the Hans mainly showed the elevated total cholesterol （TC） and low-density lipoprotein cholesterol （LDLC） compared with the controls（P<0.05）. The Uygur increased significantly in weight,body mass index（BMI） and waist circumference compared with the Hans in the insulin resistance subjects（P<0.05）. The Uygur increased significantly in body mass index compared with the Hans,the controls（26.84±4.19 kg/m² vs 25.52±3.19 kg/m²） and the insulin resistance subjects（27.77±3.60 kg/m² vs 26.09±3.68 kg/m² P<0.05）. Diabetes mellitus,hypertension,TC,and triglyceride（TG） had positive correlation with IR（P<0.01） and high density lipoprotein cholesterol（HDLC） had negative correlation with IR by Logistic Regression（P<0.01）. Conclusion The Uygur had more obesity and the Hans had higher cholesterol in the population with insulin resistance in Hospital,Urumqi. Diabetes mellitus,hypertension and dyslipidemia were closely related to IR.

Abstract:Aim To investigate the association between high density lipoprotein （HDL） subclasses and plasma triglyceride （TG） concentration in metabolic syndrome （MS）. Methods Concentrations of plasma lipids and the apolipoproteins were detected by automated biochemical analyzer. According to the NCEP ATP-Ⅲ file set by the U.S. National Cholesterol Education Program,the MS subjects were divided into four groups of TG<1.69 mmol/L,1.69 mmol/L≤TG＜2.25 mmol/L,2.25 mmol/L≤TG＜5.64 mmol/L,TG≥5.64 mmol/L . HDL subclasses were determined by two-dimensional gel electrophoresis associated with immunodetection. Results Compared with their counterpart control,the levels of plasma total cholesterol （TC）,TG,low density lipoprotein cholesterol （LDLC）,apolipoprotein B100 （ApoB₁₀₀）,preβ1-HDL,HDL_3b,ApoB₁₀₀/AⅠ and LDLC/HDLC were significantly increased in MS （P<0.05 or P<0.001）. Meanwhile,the concentrations of HDLC,ApoAⅠ,HDL_2b and HDL_2a were significantly decreased in MS （P<0.05 or P<0.001）. The content of preβ1-HDL,HDL_3a and HDL_3b were increased,while the HDL_2a and HDL_2b were significantly decreased,with the increasing of plasma TG levels in MS. Conclusion HDL subclasses distribute abnormal in MS,the content of preβ1-HDL and HDL_3b were increased,while HDL_2b and HDL_2a were significantly decreased in MS. The levels of plasma TG may be one of the influencing factors of abnormal HDL subclasses in patients with MS.

Abstract:Aim To investigate the genetic association of glutathione peroxidase-1（GPX-1） gene polymorphism in patients with kawasaki disease （KD）,and coronary artery lesions （CAL）. Methods There were 92 patients with KD and 108 healthy subjects in this study. The genotype of locus －46C/T and locus 599C/T of GPX-1 were detected by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）. Results For －46C/T polymorphism and 599C/T polymorphism in GPX-1 gene,there were no significant differences between KD patients and the controls in genotype frequencies of CC,CT and TT,and allele frequencies of C and T （χ²0.174 and 0.166,both P>0.05 χ²0.429 and 0.368,both P>0.05） For －46C/T polymorphism and 599C/T polymorphism in GPX-1 gene,there were no significant differences between KD patients with CAL and without CAL in genotype and allele frequencies of C and T （χ²0.507 and 0.487,both P>0.05 χ²0.635 and 0.535,both P>0.05）. Conclusion No association was found in －46C/T polymorphism and 599C/T polymorphism between GPX-1 gene and the risk of KD or its complication of CAL in this study.

Abstract:Aim to investigate the relationship between the average platelet volume and severity of coronary atherosclerosis. Methods 1924 adult subjects who underwent coronary angiography were enrolled in the present study. The average platelet volume of the subjects’ was measured by the automate blood analyzer,and the severity of coronary atherosclerosis was defined by the SYNTAX score. Results A significant difference of the level of average platelet volume was found among the different groups（P<0.01）. Spearman correlation analyses indicated that the SYNTAX score was significantly associated with mean platelet volume（r0.687，P<0.001）. Multiple regression analysis suggested that the level of average platelet volume was independently associated with the SYNTAX score. Conclusion The level of average platelet volume was significantly associated with the severity of coronary atherosclerosis.

Abstract:Aim To study the relationship between Ca^2＋/calmodulin-dependent protein kinase Ⅳ （CaMKⅣ） in Xinjiang Kazakh populations and patients with essential hypertension （EH）. Methods Based on case-control study,70 patients with EH and 65 subjects with normal blood pressure were selected from Hankazi township of Xinjiang where Kazakh people live relatively fixed. By physical examination and questionnaire,basic data and blood samples were collected. Serum CaMKⅣ level was detected by using double antibody sandwich enzyme linked immunosorbent assay. Results Serum CaMKⅣ level in EH group was significantly lower than that in normal blood pressure group （P<0.01）. There were no correlation between serum CaMKⅣ and total cholesterol,triglyceride,low density lipoprotein cholesterol,high density lipoprotein cholesterol （all P>0.05）. There were no correlation between serum CaMKⅣ and systolic blood pressure （SBP）,diastolic blood pressure （DBP） in the normal blood pressure group. There were negative correlation with SBP,DBP in EH group,more obviously correlated with the latter （r＝－0.291,－0.381,all P<0.05）. CaMKⅣ had more obvious correlation with patients whose diastolic blood pressure was no less than 100 mmHg （r＝－0.411,P<0.05）. Logistic regression analysis showed that higher serum CaMKⅣ had evidently protective effect to essential hypertension （OR was 0.930,95%CI was 0.909～0.952）. Conclusions Serum CaMKⅣ has independently negative correlation with EH of Kazakh populations in Xinjiang,and it is an independent factor of EH.

Abstract:Aim To explore the impact of atorvastatin on clinical efficiency and endothelial function of aged patients with unstable angina pectoris. Methods 120 cases with unstable angina pectoris were randomly divided into control group and observation group. The control group was treated by conventional therapy,the observation group received atorvastatin 20 mg additionally. The treatment duration was 12 weeks. Before and after treatment,the endothelial vasodilation function was determined by color Doppler flow imaging,fasting venous blood was collected to detect blood lipid and nitric oxide （NO）,the clinical efficiency was compared between the two groups. Results For the observation group,after treatment,the levels of total cholesterol （TC）,triglyceride （TG）,high density lipoprotein cholesterol （HDLC）,low density lipoprotein cholesterol （LDLC） and NO,the degree of brachial artery flow mediated dilatation improved greatly （P<0.05）,the clinical efficiency of angina pectoris and cardiograph was 86.7% and 76.7%,respectively there were significant differences between two groups （P<0.05）. Conclusion Atorvastatin treatment could effectively alleviate the patients with unstable angina pectoris,its treatment effect may be related to the improvement of blood lipid and endothelial function.

Abstract:Aim To investigate the clinical manifestation,diagnosis and treatment of acute viral myocarditis as acute coronary syndromes by electrocardiogram （ECG） characteristics. Methods Retrospective analysis was carried out for the data of 20 patients adimitted to Shanghai First People’s Hospital from June 2008 to June 2014. These patients had been diagnosed as acute viral myocarditis,but looking as acute coronary syndromes. Among these patients,there were 12 males and 8 females,aged 17～60 years,mean 38 years,and 6～14 days hospitalization. Results On admission,all 20 patients showed significant higher markers of myocardial necrosis,and their ECG’s ST-segments were arched elevation in the inferior wall or front wall. During hospitalization,these patients were excluded from acute myocardial infarction by coronary angiograph or coronary computed tomography angiography （CTA）. Meanwhile,they were diagnosed as acute viral myocarditis by cardiac magnetic resonance,endomyocardial biopsy,and 4 cases were diagnosed as acute severe viral myocarditis. By applying heart failure treatment,myocardial nutrition,reducing myocardial oxygen consumption,angiotensin converting enzyme inhibitor and other comprehensive treatment,19 cases were cured,and 1 case of acute fulminant myocarditis was dead. Conclusion Part of acute viral myocarditis patients resemble the ECG characteristics and manifestations of acute coronary syndrome,and is easy to be misdiagnosed. It is necessary to combine patient history,clinical manifestations,ECG,coronary angiography,cardiac enhanced magnetic resonance examination to make timely diagnosis.

Abstract:Statin therapy has been proven successful in the primary prevention of acute coronary syndrome （ACS）.

Abstract:Percutaneous coronary intervention （PCI） is one of the important means of treatment of coronary heart disease,early PCI treatment can restore a stable coronary blood flow,reduce infarct size,thereby improve prognosis and reduce case fatality rate. However PCI causes mechanical vascular injury,the Stent and Balloon dilatation causes endothelial denudation,intimal tear,activate the excessive proliferation of smooth muscle cells and platelet adhesion and aggregation,leading to stenosis and thrombosis. Drug-eluting stent Restenosis was reduced to a certain extent,but could cause to delay endothelialization and lead to late stent thrombosis formation. Bone marrow and peripheral blood endothelial progenitor cells （EPC） involvement of endothelial and angiogenesis,in atherosclerotic lesions may play an important role in the development process,this paper discusses the role,characteristics,mechanism and prediction mechanism and therapeutic aspects of EPC to the repair function of endothelial injury in patients with CHD after PCI.

Abstract:Plasma DNA and mitochondrial DNA are circulating DNA in the blood. The healthy has extremely small amounts of them,but in pathological state,the body often has a different increase in amount. Through real-time monitoring of plasma DNA and mitochondrial DNA,myocardial infarction and cardiac arrest can achieve early diagnosis and prognosis.