Abstract:Aim To analyze the relationship between the level of receptor for advanced glycation endproducts （RAGE） on peripheral blood mononuclear cells （PBMC） and the clinical manifestations of coronary heart disease and the severity of angiographic lesion. Further investigation should be made to discuss whether RAGE can play a guiding role in the prediction and assessment of the risk of coronary heart disease. Methods 80 study participants were scheduled to undergo elective cardiac catheterization for suspected coronary artery disease according to clinical manifestations of coronary heart disease, Gensini scores of coronary angiographic stenosis and the number of diseased coronary artery, all subjects were divided into different groups. Expression of RAGE on PBMC was detected by flow cyto-metric analysis. Results

Abstract:Aim Take coronary heart disease patients peripheral blood mononuclear cells (PBMC)as the study object, observing the effect of niacin to the expression of cyclic adenosine monophotosphate (cAMP)and adenosine triphosphate binding cassette transporter A1 (ABCA1) in PBMC which incubated in vitro, analysing the correlation of niacin adjusts the expression of ABCA1 and cAMP/ protein kinase A (PKA) pathway. Methods To detect the expression of ABCA1 mRNA and protein by reverse trancriptase-polymerase chain reaction (RT-PCR) and western blot, to determine the level of cAMP in PBMC by low pH EIA kit. Analysing the expression of ABCA1 change and cAMP level change, discuss the correlation on the expression of ABCA1 and cAMP in PBMC by the role of niacin. Results (1)Each group of the expression of ABCA1 and the cAMP level changes in PBMC in 3 h and 48 h does not have the obvious difference (p<0.05). (2)The expression of ABCA1 of coronary heart disease high blood fats patient in PBMC compares the normal person obviously to reduce (p<0.05), but in the normal blood fats group the expression of ABCA1 in PBMC compares the normal person not to have the obvious change (p<0.05). (3)The niacin alone affects obviously increase in coronary heart disease person uninuclear cell the ABCA1 expression (p<0.05), at the same time makes the cAMP content in the PBMC to increase (p<0.05). (4)PKA inhibitor Ro 31-8220 alone affects can obviously reduce in coronary disease person he expression of ABCA1 in PBMCs (p<0.05),at the same time causes the cAMP content in PBMCs to reduce (p<0.05). (5)In the niacin +PKA inhibitor group were preincubated for 30 minutes in the presence of PKA inhibitor Ro 31-8220 (900 nmol/L), before niacin (1 000μmol/L)was added. then the niacin increases the expression of ABCA1 and the cAMP content in PBMCs was suppressed completely (p<0.05). Conclusion According to the result analysis of the experiment, the effect that niacin affect the expression of ABCA1 through the pathway of cAMP/ PKA, and there is an explicit correlation that niacin increase the expression of ABCA1 in PBMCs with the pathway of cAMP/PKA.

Abstract:Low density lipoprotein receptor(LDLR) mRNA in peripheral blood mononuclear cells(PBMC) belong to low abundance expression and is difficult to detect.This study found that using the reverse transcription(RT)-polymerase chain reaction(PCR)procedure for semiquantitative determinating expression levels of LDLR gene is a highly sensitive and specific,and gives a convinient and necessary method for clinical studying expression levels of LDLR gene in PBMC from patients with hypercholesterolemia and coronary heart disease.