Abstract:Aim To observe the protective effects of bone marrow mesenchymal stem cells transplantation for treatment of cerebral ischemia injury in rats,and investigates the expression of interleukin 10 (IL-10) and transforming growth factor beta 1 (TGF-β1) to explore its possible mechanism of nerve repair. Methods 36 male SD rats were randomly divided into blank group, model group,and transplantation group. MCAO model was formed by suture occluded method. Rats intransplatation group were given Bone marrow mesenchymal stem cells. Rats in model group were given the same dose of normal saline and nothing were given to blank group. Evaluate their condition by Bederson scoring at 1,3,7,4 days after injured. Area of cerebral infarction was measured through brain tissue TTC staining. Detect the changes of inflammatory factors IL-10 and TGF-β1 in serum and brain tissue of rats by ELISA and Western blot. Results The blank group had low expression of IL-10 and TGF-β1 . Compared with the blank group, both the volume of cerebral infarction of rats in the model group and the scores of the nervous function behavior were increased markedly (P<0.01), the expression of IL-10 and TGF-β1 in serum and brain tissue was significantly higher than that in the blank group(P<0.05). Compared with the model group, the cerebral infarct volume in the experimental group was decreased , the scores of the nervous function behavior of rats were significantly decreased at 3,7,14 days (P<0.05), the expression of IL-10 and TGF-β1 in serum and brain tissue was significantly higher than that in the model group (P<0.01). With the passage of time, the expression of IL-10 and TGF-β1 was gradually reduced. Conclusions BMSC transplantation is able to improve the recovery of the cerebral ischemic injury. The mechanism may be related to the secretion of anti-inflammatory cytokines IL-10 and TGF-β1.

Abstract:AimTo discuss clinical efficacy and safety of symptomatic middle cerebral artery (MCA) stenosis Wingspan stent placement.MethodsThe retrospective analysis includes 23 cases of symptomatic MCA acute ischemia and medical treatment-failed MCA M1 stenosis receiving self expandable Wingspan stent and 8 clinical cases of MCA M1 stenosis acute ischemia which enjoy stent treatment indications but receive medical treatment instead of stent treatment for economic reasons.Stent group receives Gateway sacculus pre-expansion and then is implanted with self expandable Wingspan stent.Medicine group receives edaravone injection and salvia miltiorrhiza needle and effect evaluation 7, 14 and 21 days after the operation and group’s clinical curative effect is then analyzed.ResultsThere is significant difference between the two groups 14 and 21 days after treatment in improvement of neural function and daily life activities.ConclusionsWingspan self expandable stent placement on symptomatic M1 stenosis is feasible, safe and effective.Recent follow-up visits have confirmed its curative effects in effectively improving neurologic impairment resulting from acute cerebral infarction and daily life ability.But it is a high-technical wounded treatment.Therefore indications must be strictly treated to prevent abuse for sake of security.

Abstract:Aim To observe the expression of brain-derived neurotrophic factor (BDNF) and Semaphorin 3A(Sema 3A) after focal cerebral ischemia,and to study the neuroprotective effects of puerarin. Methods The model of focal cerebral ischemia was established by occluding middle cerebral artery(MCAO).Dynamic changes of BDNF and Sema 3A positive neurons at different time were observed with method of immunohistochemistry. Results The number of BDNF positive neurons increased after 6 h of ischemia,and reached its peak at the time of 1 d after ischemia.Compared with treatment group and ischemic group,the levels of BDNF were higher(p<0.05);the number of Sema 3A positive neurons increased after 6 h of ischemia,and reached its peak at the time of 1 d after ischemia,then become normal after 3 d.Compared with treatment group and ischemic group,the levels of Sema 3A were lower(p<0.05). Conclusions After the attack of cerebral ischemia, the expression of BDNF and Sema 3A may be related to the mechanism of neuronic injury and repairment.Puerarin may have protective effects on ischemic neurons.

Abstract:Aim To study the change of the interleukin-8 (IL-8) content in blood and cerebrum after cerebral ischemia-reperfusion injury. Methods With Zea Longas thread method, cerebral ischemia-reperfusion injury model of rats was established. The rats were randomly divided into the groups as sham operated control group and ischemia-reperfusion 3 h, 6 h, 12 h, 24 h and 48 h groups. The content of IL-8 in blood serum and brain tissue was detected by double antibody sandwich enzyme-linked immunosorbent assay. Results The content of IL-8 in blood serum was increased significantly at 3 h after reperfusion (p<0.01), and then went down. It reached the level of the control group at 24 h after reperfusion. The concentration of IL-8 in brain tissue was peaked at 6 h after reperfusion (p<0.01), went down after 12 h reperfusion. Moreover, there is no difference between the group of 6 h after reperfusion and 12 h after reperfusion (p>0.05). Conclusion IL-8 was involved in the pathophysiological course of cerebral ischemia-reperfusion injury.

Abstract:Aim To investigate the changes of transcranial doppler (TCD) in mind-body relaxing therapy (MBRT) of patients with cerebral arteriosclerosis (CAS). Methods 60 CAS patients were randomly divided into mind-body relaxing therapy with routine treatment group (n=30) and routine treatment group (n=30) for the treatment of 8 weeks. The clinical effects were assessed by TCD. Results Compared with before treatment, Vm, Vs, and Vd of base artery (BA) and left middle cerebral artery (LMCA )were significantly increased, RI and PI were significantly decreased in mind-body relaxing therapy with routine treatment group after treatments of 8 weeks (p<0.05 or p<0.01); the significant rate and total effctive rate of TCD frequency spectrum in mind-body relaxing therapy with routine treatment group were higher than routine treatment group (p< 0.05 ). Conclusion The mind-body relaxing therapy can significantly improve the blood flow velocity, reduce the resistance of cerebral blood vessel, and increase therapeutic effect of the patients with CAS. Mind-body relaxing therapy with routine treatment group has better effect in treatment of CAS than routine treatment group.

Abstract:Aim To study the change of interleukin-10 (IL-10) in focal ischemia tissue of brain and serum in middle cerebral artery occlusion (MCAO) of rats. Methods The middle cerebral artery (MCA) was occluded for different time with modified Zea Longa s MCAO model. The level of interleukin-10 in focal ischemia tissue of brain and blood serum was measured with double-antibody sandwich enzyme linked immunosorbent assay (ELISA), both in ischemia group and in control group. Results The content of interleukin-10 in focal ischemia tissue of brain was lower at 3 h after ischemia, compared with the control (P> 0.05), and it fell to the lowest degree at 6 h, compared with the control (P< 0.01), then it increased compared with the control (P< 0.05). The concentration of interleukin-10 in serum fell to the lowest degree at 3 h after ischemia compared with the control (p<0.05), and then it increased slowly compared with the control (p< 0.05). Conclusion Interleukin-10 perhaps afford protection to the cerebral ischemia injury.

Abstract:Aim To observe the characteristic of intracranial large arterial lesions that were induced by experimental hypertension.Methods A morphometric study was performed on middle cerebral artery and basal artery in stroke-prone renovascular hypertensive rats and the morphometric values afert antihypertensive therapy with captopril were compared with those in non-antihypertensive rats.Results It was found that enhanced media thickness was presented in both middle cerebral artery and basal artery. It was mainly induced by hypertrophy and hyperplasia of smooth muscle cells in middle cerebral artery and by mural remodeling in basal artery. The hypertrophy and hyperplasia of smooth muscle cells were depressed, but the remodeling did not reverse to be sufficient to bring about full normalization after antihypertensive therapy.Conclusion These results indicate that the main lesion of intracranial arteries is enhanced media thickness, but different vessels have different reaction to hypertension and antihypertensive therapy.