Abstract:Aim To investigate the expression and correlation of nuclear factor κB (NF-κB), Toll-like receptors (TLR) and inflammatory cytokines in the rupture of intracranial aneurysm (IA). Methods The IA and IA ruptured aneurysm tissues were collected by surgery as the IA group and the IA rupture group, and healthy vascular tissues were collected as a control group. The levels of NF-κB, TLR4, interleukin 1β (IL-1β) and hypersensitive C-reactive protein (hs-CRP) mRNA in aneurysm tissues were detected by qPCR. NF-κB and TLR4 protein levels were detected by Western blot. The levels of IL-1β and hs-CRP in the serum of each group were detected by enzyme-linked immunosorbent assay.Results The levels of NF-κB, TLR4, IL-1β and hs-CRP mRNA and protein in the aneurysm of IA group and IA rupture group were significantly higher than those of the control group (P＜0.05). The levels of NF-κB, TLR4, IL-1β and hs-CRP mRNA and protein in the aneurysm of IA rupture group were significantly higher than those of IA group (P＜0.05). Serum IL-1β and hs-CRP were significantly higher in the IA group and the IA rupture group than in the control group (P＜0.05). The levels of serum IL-1β and hs-CRP in the IA rupture group were significantly higher than those in the IA group (P＜0.05). Conclusion Compared with IA patients, IA-ruptured patients have higher levels of NF-κB, TLR4, IL-1β, and hs-CRP mRNA and protein expression in aneurysms, suggesting that NF-κB, TLR4, IL-1β, and hs-CRP are involved in IA rupture.

Abstract:Aim To compare the effects of morning versus evening intake of simvastatin on lipid profile and high-sensitivity C-reactive protein(hs-CRP) in patients with combined hyperlipidemia.Methods103 patients were randomly assigned to receive 20 mg simvastatin orally each morning(n=52) or evening(n=51).The treatment period lasted 6 months.Lipid profiles,physical and laboratory investigations for adverse effects,and hs-CRP were assessed.ResultsSerum total cholesterol(TC),low density lipoprotein cholesterol(LDLC) and triglyceride(TG) levels decreased significantly in both treatment groups,while simvastatin in the morning administration resulted in larger reductions in TG than dose evening administration(38% vs 19%,P=0.035).Moreover,morning administration reduced TC and LDLC by 21% and 31%,respectively.The success rates of TG and all three together(TC,LDLC and TG) were 51% and 36% in the morning administration,which were superior to the drug given in the evening(33% and 29%,respectively).Although serum hs-CRP levels decreased significantly from baseline in both morning and evening administration,similar hs-CRP reductions were observed between the two treatment groups(p<0.05).And the effects were cholesterolindependent.There were no adverse events during the treatment periods,and simvastatin was well tolerated in the morning or evening administration.ConclusionsThe results demonstrated that TG lowering effects of simvastatin in the morning administration were superior to that of evening administration.There is no diurnal variation in anti-inflammatory effects of this drug,therefore,morning or evening administration is equally effective in decreasing hs-CRP.