云芝多糖对受氧化型低密度脂蛋白攻击的小鼠腹腔巨噬细胞泡沫样变性及衰减的影响
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Effect of Polysaccharide on Foam-like Change and Attenuation of Murine Peritoneal Macrophages Attacked by Oxidized Low Density Lipoprotein
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    摘要:

    以氧化修饰低密度脂蛋白攻击小鼠腹腔巨噬细胞为损伤模型,观察了云芝多糖对巨噬细胞泡沫样变性及衰减的影响的时效和量效关系。腹腔注射云芝多糖的小鼠腹腔巨噬细胞在体外与10、50、100mg·L-1等三种浓度的氧化型低密度脂蛋白共同培养24、48和72h后,细胞形态未有较大改变,用MTT比色法计数各时间点细胞存活数,受云芝多糖保护的巨噬细胞存活数较高。结果表明,云芝多糖能抑制巨噬细胞泡沫样变性,以及巨噬细胞泡沫样变性后,云芝多糖能延长其生存时间,防止其死亡,这对抑制动脉粥样硬化的进程有益处。

    Abstract:

    With mimicking the injury model from murine peritoneal macrophages attacked by oxidized low density lipoprotein (OLDL), the time-de pendant and concentration-depandant effect of polysac charide K (PSK) on the foam-like change and attenuation of murine peritoneal macrophages attacked by OLDL was observed.After incubated with 10,50 and 100 mg·L-1 OLDL for 24,48,72 hOurs in vitro, the fingure of PSK-treated macrophages was maintained normal relatively.Moreover,in PSK-treated group, the amount of survival cells were higher with counted by MTT colorimetric analysis at every time points.So PSK could inhibit the foam-like change of macrophages efficiently.And after the change in macrophages formed,PSK could prolong the survival time of these macrophages.

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娄宁,周玫,陈瑗.云芝多糖对受氧化型低密度脂蛋白攻击的小鼠腹腔巨噬细胞泡沫样变性及衰减的影响[J].中国动脉硬化杂志,1995,3(4):287~290.

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  • 收稿日期:1995-07-30
  • 最后修改日期:1995-11-19
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