肥胖儿童血清脂蛋白(a)水平及载脂蛋白(a)的遗传表型研究
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山西省科委资助项目


Studies on Sera Lipoprotein(a) Levels and Genetic Phenotypes of Apolipoprotein(a) in Obese Children
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    摘要:

    本文对肥胖儿血清脂蛋白(a)水平及血清载脂蛋白(a)多态性进行分析,以探讨肥胖儿与高脂蛋白(a)血症的关系及其血清载脂蛋白(a)遗传表型的分布特点。用酶联免疫吸附法测定57名肥胖儿及其双亲血清脂蛋白(a)的含量,用十二烷基磺酸钠一聚丙烯酰胺凝胶电泳和Westernblot免疫印迹技术测定载脂蛋自(a)遗传表型。结果发现高脂蛋白(a)血症的肥胖儿中约有75%的患儿其父母中有一方伴发高脂蛋白(a)血症;在41名肥胖儿中共检出7种载脂蛋白(a)遗传表型(其中5种单异构体表型,2种双异构体表型).肥胖儿中以低分子量的S1和S2表型为主;非肥胖儿以高分子量的S4在型为主,在近5年文献一尚未见类似报道。

    Abstract:

    This report presents the studies ofsera levels of lipoprotein(a) [Lp (a)] and genetic polymorphism of apolipoprotein(a)[Fapo(a)] in obese children. The aims of this study were to explore the relationship between obese children and high sera levelsof Lp(a) and the distributive characteristics of geneticphenotypes of apo(a).Enzyme-linked immunosorbent assay(ELISA) was used to measure sera levels of Lp(a)in 57 obese children and their parents. The genetic phenotypes of apo (a) were determined by SDS-polyacrylamide gel electrophoresis(SDS-PAGE)and Western blot analysis, Of 75%subjects of obese children with high Lp(a) level, one side of their parents had high concentration of sera Lp(a).A total of 7 different phenotypes of apo(a) were determined from 41 obese children , including 5 different phenotypes of single-isoform and 2 different phenotypes of two-isoform,Obese children mostly had S1 and S2 phenotypes,but the controls generally had S4 phenotype.The similar reports have not been found in the recent5-year references. The study of genetichenotypes of apo(a) in obese children can be of great importance to protection and treatment of the cardiovascular and cerebrovascular disease induced by obesity.

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刘德文,候泽,张悦红,陈红杰,吕华,谢瑞瑝,王美德,赵吉娥,武纯,李瑞畸,强梅.肥胖儿童血清脂蛋白(a)水平及载脂蛋白(a)的遗传表型研究[J].中国动脉硬化杂志,1996,4(4):255~259.

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  • 收稿日期:1996-10-14
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