糖基化低密度脂蛋白增强人外周血单核巨噬细胞的Sis基因表达
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国家自然科学基金


Enhancement of sis Gene Expression in Human Monocyte-macrophages by Glycosylated Low Density Lipoprotein
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    摘要:

    目的探讨体外糖基化低密度脂蛋白对人单核巨噬细胞中sis基因表达的影响及其在糖尿病易患动脉粥样硬化中的意义。方法密度梯度离心分离并培养人外周血单核巨噬细胞,提取总RNA,地高辛标记sis基因探针和斑点杂交后化学发光检测及光密度扫描。结果糖基化低密度脂蛋白显著增强人外周血单核细胞sis基因表达。结论糖基化低密度脂蛋白对人外周血单核细胞sis基因表达的作用是糖尿病易患动脉粥样硬化的重要机制。

    Abstract:

    Aim To investigate the effect of glycosylated low density lipoproteins(gLDL)on expression of sis oncogene in human monocyte-macrophagesand its significance in accerelated atherosclerosis associated with diabetes.Methods Total RNA were isolated from monocyte-macrophages which were prepared by density gradient centrifugation from healthy subjects blood,The dot blot hybridizations were performed with digoxin labelling V-sis probes and detected by chemical lu minescence and densitometry scanning,Results The gLDL significantly enhanced the expression of V-sis in cultured human monocytemacrophages.Conclusions The stimulating effects on sis oncogene expression of gLDL contribute to its atherogenic properties, which may be an important machanism for high incidence of atherosclerosis in diabetic patients.

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刘乃丰,黄晓明,胡向阳,沈成兴,张丽容.糖基化低密度脂蛋白增强人外周血单核巨噬细胞的Sis基因表达[J].中国动脉硬化杂志,1996,4(4):291~293.

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  • 收稿日期:1996-09-09
  • 最后修改日期:1996-12-10
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