Aim The present pater is to study the effect of different 3'end of variable number of tandemly repeat (VNTR) alleles from human apoliopoprotein B gene on eukarytic expression and regulation. Methods HVE36 allele distributed widely in nor- mal Population and HVE44 allele distributed mainly in patients with atherosclerosis were cloned into plasmids of luciferase reporter vecters pGL2-promoter and PGL2-control. Then the recombinants were transfect- ed iuto Hela and HepG2 cell lines.Results The level of expression of pGL2-control plasmid inserted with WE44 allele was 4 times than those of the recombinant with HVE36. The level of expression of pGL2-promoter plasmid inserted by HVE44 is 1. 7 times than those in plasmid inserted by HVE36 allele in Hela cell line, In HepG2 cell line, the level of expression of pGL2-control recombinant with HVE44 is 3. 05 times of those in HVE36 recombi- mant. But in PGL2-promoter system, the level of expression of recombinant with HVE44 alleles is 2.1 time than those in HVE36 recombinant. In differ- ent cell lines, the expression level of recombinant with HVE44 was much higher than those of HVE36 recom- binant. Conclusioas Different 3'VNTR alleles have regula-tory role in gene expression and HVE44 allele can pos-itively regulate the eukarytic expression, which may be one of causes of higher apolipoprotein B level in atherosclerosis patients with VNTR-big alleles like HVE44. More investigations are carried out for regu- latory mechanism of the 3'-end nontranslation region.