Aim To determine the effects of Liensinine on endothelin-stimulated proliferation of vascular smooth muscle cells (VSMC) and expression of heat stress protein (HSP70) and antioncogene P53. Metbods The experimental models of proliferation of cultured porcine aortic smooth muscle cells induced by endothelin were established, and H-thymidine(~3 H-TdR ) incorporation, flow cytometry, Western blot and Northern blot analysis were used.Rerults Liensinine may drop H-TdR incorporation induced by endotbelin and hold-back VSMC from G0/ G, phase to DNA synthetic phase (S) and mitotic phase (G2/M ). Furthermore, Liensinine could reverse the enkanced expression of HSP70 and HSP70 mRNA and reduced expression of antioncogene P53 induced by endothelin.Conclusions Liensinine may inhibit DNA synthesis and proliferation of VSMC, related to the mechanism of molecular biology of controlling HSP70 and P53.