脂质过氧化对培养的内皮细胞表达细胞粘附分子的影响
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Effect of Lipid Peroxidation on the Expression of Cellular Adhesion Molecules in Cultured Endothelial Cells
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    摘要:

    为观察脂质过氧化损伤对细胞粘附分子表达的影响,用联胺诱发培养的人血管内皮细胞脂质过氧化后,检测其丙二醛含量及单核细胞粘附率,并用激光共聚焦显微镜观察细胞粘附分子表达的情况。结果发现,实验组较对照组内皮细胞丙二醛含量升高,单核细胞粘附率显著增加(P<0.01),内皮细胞上有血管细胞粘附分子-1及内皮细胞白细胞粘附分子-1的表达,且其表达量均随时间增多。提示内皮细胞粘附分子的表达增加可能是脂质过氧化损伤导致单核细胞粘附增多的重要机制。

    Abstract:

    Aim: To explore whether lipid peroxidation (LPx)is capable of inducing the expression of cellular adhesion molecules (CAMs ) to increase monocyte (MC )adhesion to endothelial cells (EC).Methods: LPx in EC was initiated with diamide (DM), the content of lipid peroxide (LPO) in EC and MC adherence were rneasured. After the EC was stained with irnmunofluorescence, the expression of vascular cell adhesion rnolecule (VCAM-1 ) and endothelial leukocyte adhesion moIecule (ELAM-1 ) was studied under laser confocal microscopy.Results: After LPO content in EC increased, EC expressed VCAM-1 and ELAM-1. The expression of VCAM-1 and ELAM-1 was upregulated in a time-dependent way (1, 2, 4 and 8 h ) and correlated with a significant increase of MC adherence to EC.Conclusion: LPx could induce the expression of CAMs in EC to enhance adherence of MC to EC and migration of MC into subendothelial space which is crucial for initiation and development of atheroscleroticles ions.

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尹鸿操,陈铁镇,董玉兰,杨向红,洪伟.脂质过氧化对培养的内皮细胞表达细胞粘附分子的影响[J].中国动脉硬化杂志,1998,6(2):120~123.

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  • 收稿日期:1997-07-17
  • 最后修改日期:1998-04-20
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