Aim To establish a immunocytochemistry method of detection to thrombin receptor(TR).Methods TR activated by thrombin can release a peptide that contain 41 amino acids(TR1-41)from N-terminal of TR.The TR1-41 was synthesized accord-ing to the amino acids sequence of TR,then mice were immunized utilizing the TR1-41,4 cell strains produc-ing TR-McAb were gotten.Preparations of atheroscle-rotic human left ventricular myocardium of four died patients were cut out.The preparations were made in-to paraffin sections.Results A immunocytochemistry method-alkaline phosphatase antialkaline phosphatase(APAAP)to de-tect the thrombin receptor was established and the TR of myocardium were detected successfully.Conclusion The method established can detect TR expression of tissue and organ.