Aim To investigate the effect of bovine aortic heparan sulfate proteoglycan (HSPG) ,chondroitin sulfate proteoglycan(CSPG) and dermatan chondroitin sulfate proteoglycan (DSCSPG) on the growth of cultured human umbilical vein endothelial cells (hUVEC). Methods PGs were isolated by dissociative extraction and column chromatography from bovine aortic intima media and their effects on the growth of cultured first passaged hUVEC was studied by 3 H TdR incorporation and cell count. The first passaged hUVEC (2.5×10 4) were planted onto 24 well plant (5 wells or 3 wells for each PG) and cultured by M 199 containing 15% HS and 10% FCS for 24 h, and then the hUVEC were incubated with M 199 containing 15%HS, 10%FCS and different concentrations of PGs (PGs were presented as hexuronic acid) respectively together with controls without PG for 24 h. Results The inhibiting percentages of proliferation (based on 3H TdR incorporation and cell count ) with HSPG (hexuronic acid 2.1～8.3 mg/L) were 28.3%、52.3% and 75.4%(21.5%、37.8% and 62.9%), with CSPG (hexuronic acid 9.5～37.8 mg/L) were 61%、83% and 91%(37.2%、68.9% and 76.5%) and with DSCSPG (hexuronic acid 9.3～36.9 mg/L) were 58.4%、82.4% and 86%(44.5%、61.6% and 71.7%) Conclusion bovine aortic HSPG, CSPG and DSCSPG could inhibit the proliferation of hUVEC and the inhibitions were associated with the PG concentrations. 还原