消斑肽对C57BL/6J小鼠动脉粥样硬化斑块的影响
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XBP-01 Eliminates Atherosclerotic Plaques on C57BL/6J Mice Fed with Atherogenic Diets
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    摘要:

    已发现从中华眼镜蛇毒素中提取的一种多肽( 暂命名为消斑肽) 能抑制血管平滑肌细胞增殖,并在一定范围内杀伤快速增殖的平滑肌细胞。为验证消斑肽是否具有消除动脉粥样硬化斑块和预防再狭窄的药物效应,选用动脉粥样硬化敏感株近交系C57 B L/6 J小鼠, 经致动脉粥样硬化饮食饲喂17 周。使用消斑肽腹腔注射3 .2 μg/g 治疗4 周,在主动脉窦部连续切片,油红O 染色并在显微镜下观察,使用Roberts & Thompson 评分判定斑块消退情况。结果发现致动脉粥样硬化饮食饲喂17周后,主动脉窦形成典型的动脉粥样硬化斑块,且血清总胆固醇和甘油三酯大幅度升高,约高于对照组三倍。经消斑肽治疗后小鼠斑块明显少于和小于非治疗组, 两组的Roberts & Thompson 评分分别为4 .1 ±2 .2 和8 .0 ±1 .2 ( n = 10 ,P< 0 .05) ;而且血清总胆固醇和甘油三酯均下降,接近对照组,非治疗组则下降不明显。此结果提示消斑肽对于小鼠动脉粥样硬化斑块的形成有一定的阻抑作用,且对已形成的斑块有消退作用;同时能使血清总胆固醇和甘油三酯下降。

    Abstract:

    Aim Previous studies performed in vitro indicated that XBP-01, a polypeptide which was refined from Chinese cobra venom (Naja naja atra), could inhibited the cultured vascular smooth muscle cell proliferation. To observe the eliminating atherosclerotic plaques effect of XBP-01 in vivo. Methods After fed with the atherogenic diets for 17 weeks, the atherosclerotic sensitive inbred strain C57BL/6J mice have typical atherosclerotic plaques formed near the aorta sinus, accompanied with the serum total cholesterol and triglyceride significant rise. Then the mice were injected with XBP-01 of 3.2 μg/g weight body per day for 4 weeks. Cryostat frozen sections, 10 μm thick, were cut from heart to aorta, stained with Red oil O. The sections were examined microscopically and scored according to the method of Roberts and Thompson. The serum total cholesterol and triglyceride also were assayed. Results The mice have typical atherosclerotic plaques formed near the aorta sinus, accompanied with the serum total cholesterol and triglyceride significant rise after fed with the atherogenic diets for 17 weeks. The plaques are fewer and less than that of the non-treating with XBP-01 group, the Roberts & Thompson score is 8.0±1.2, 4.1±2.2, respectively, and has a statistical significance. The serum total cholesterol and triglyceride descends to normal level. Conclusions XBP-01 possesses the potential antiatherosclerotic effect in vivo.

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袁中华,杨永宗,杨小毅,谭健苗,万载阳.消斑肽对C57BL/6J小鼠动脉粥样硬化斑块的影响[J].中国动脉硬化杂志,1999,7(3):215~218.

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  • 收稿日期:1998-06-26
  • 最后修改日期:1999-08-09
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