Aim To study the relationship of very low density lipoprotein receptor (VLDLR) structure and its function. Methods The present paper reported the cloning of VLDLR gene cDNA from a chinese heart tissue by RT-PCR technology. A recombinant named pCD-VR was constructed in which the VLDLR cDNA was inserted a vector pcDNA3. Results Sequencing result confirmed that the chinese oriented VLDLR gene cDNA was in line with previously one. Then, the pCD-VR was transfected into CHO cells. Lipoprotein combination test and mRNA detection confirmed that this human VLDLR gene could express effectively in the CHO cells. Conclusion It was provided a new idea about the roles of VLDLR in atherosclerosis.