Aim To explore human interleukin-17 (hIL-17)gene in the pathogenesis of coronary disease. Methods Reverse transcription polymerase chain reaction (RT-PCR) was used to amplified hIL-17 gene from activated peripheral blood mononuclear cells (PBMC), hIL-17 gene was confirmed by DNA sequencing. Expression of hIL-17 mRNA in PBMC was determined by dot hybridization techniques. Serum levels of soluble intercellular adhesion molecule-1 (sICAM-1) was determined by enzyme linked immunosorbent assay (ELISA) method. Results With reference to the published sequence of the human IL-17 gene, a pair of DNA primer were designed and synthesized. A 473 bp fragment encoding fall length of hIL-17 cDNA gene was successfully amplified by using RT-PCR, and then cloned into pUC18 vector. The recombinant plasmid was sequenced to confirm hIL-17gene. Meanwhile, Using random-prime synthesis of probe for hIL-17 gene. We apply for dot hybridization techniques to determine the over expression of hIL-17 mRNA in peripheral blood monocytes (PBMC) with coronary disease, and their high levels of sICAM-1. Conclusions hIL-17 gene may play an important role in the pathogenesis of coronary Disease. Our experiment also lay a profound foundation for further analysis of hIL-17 biological functions.