Aim To investigate the lipoprotein lipase gene HindⅢ polymorphism associated with Chinese endogenous hypertriglyceridemics (HTG). Methods Lipoprotein lipase gene Hind Ⅲ polymorphism was studied using polymerase chain reaction (PCR) and restriction fragment length polymorphisms (RFLP) in 200 endogenous hypertriglyceridemics and 202 healthy subjects from a population of Chinese Han nationality in Chengdu area. Results The results showed that both in HTG group and control group, the H2H2 homozygous was the major alleles. H2 allelic frequencies in HTG group were higher than that of control group (0.855 vs 0.745,P<0.01).but H1 allelic frequencies in HTG group was significantly lower than that of control group (0.145 vs 0.255,P<0.01). the plasma triglycerides(TG) level, apo CⅡ and apo CⅢ levels, TG/HDLC ratio in H2H2 genotypes were significantly higher than that of H1H1 genotypes (P<0.01), also higher than that of H1H2 genotypes (P<0.05). Conclusions Therfore, it is suggest that the HindⅢ RFLP at intron 8 of LPL gene is associated with endogenous hypertriglyceridemics to some extent in Chinese population.