Aim To explore the correlation between TTTTA Pentanucleotide repeats(PNR) polymorphism of the apolipoprotein(a) gene and atherosclerosis cerebral infarction(ACI) in Chinese Han nationality,235 subjects with no blood links in Hubei region, including 82 patients with ACI and 153 healthy controls were studied. Methods Polymerase chain reaction (PCR) denature polyacrylamide gel electrophoresis silver stain technique was employed to determine the TTTTA PNR polymorphism of apo(a) gene. And (TTTTA) 5 allele of the apo(a) PNR was cloned into a plasmid T vector. The recombinant PGEM apo(a) was sequenced. Results The Chinese Han nationality in Hubei region has apo(a) gene polymorphism,7 alleles with 4,5,7,8,9,10,11 TTTTA PNR and 11 genotypes with 4/8, 4/9, 5/8, 5/9, 7/8, 8/8, 8/9, 8/10, 8/11, 9/9 and 9/10 was detected; the allele frequency in ACI with 5 repeats was remarkably higher than that in controls(P<0.05),the frequency in ACI with 9 repeats was remarkably lower than that in controls(P<0.01); sequence mutation of the apo(a) PNR(TTTTA) 5 was not found. Conclusions It was possible that the copy number of the apo(a) PNR (TTTTA) was associated with the susceptibility to ACI .