Aim To elucidate the relationship of apolipoproteins E gene polymorphism to coronary artery disease. Methods Apolipoproteins E genotype was analyzed in 60 patients judged by selective coronary angiography and 50 cases with negative coronary angiography as control. PCR-restriction fragment length polymorphism methods was employed using genomic DNA from anti-coagulantion blood. χ2 test and Logistic regression were used for statistical analysis. Result and Conclusion The apolipoproteins E₂/3, E3/3, E3/4, E₂/4, E4/4 and E₂/2 genotypes and ε2, ε3 and ε4 alleles frequency in patients group were 11.7%, 68.4%, 10.7%, 1.7%, 1.7%, 0 and 7%, 82.2%, 10.8%;while 6%, 76%, 16%, 2%, 0, 0 and 4%, 87%, 9% in control group respectively. χ2 test and Logistic regression analysis showed no differences in gene polymorphism between patients and control group. The apolipoproteins E gene polymorphism were also found not related to the severity of coronary artery disease.