Aim To investigate the effect of platelet derived growth factor β-receptor (PDGFR-β)antisense oligonucleotides (AODN) on platelet derived growth factor (PDGF)induced migration of cultured rat vascular smooth muscle cells(VSMC).Methods In vitro cultured rat VSMC model was established. Interactive Laser Cytometer (ILC) was used to investigate fluoresceinisothiocyanate labeled PDGFR-β AODN cellular uptake and localization. The migration of VSMC was assayed by a modification of Boyden's chamber method. Results Fluorescent AODN localized in the cytoplasm of VSMC at 24 hours and in the nucleus at 48 hours. Compared with control group, PDGFR-β AODN at dose of 5 μmol/L or higher can significantly decrease the number of cells which had migrated through the polycarbon filter induced by PDGF. PDGFR-β AODN at dose of 2.5 μmol/L or lower, sense oligonucleotide and scrambled oligonucleotide could not significantly inhibit PDGF induced migration of VSMC. Conclusion PDGFR-β AODN can localize in the nucleus and significantly inhibit PDGF induced migration of cultured VSMC in a dose and time-dependent fashion.