Aim To investigate the relation between the expression of lipoprotein lipase mRNA and the different clinical class or severity of coronary atherosclerosis. Methods A total of 34 patients with coronary atherosclerosis and 10 healthy subjects underwent coronary angiography. There were 12 patients with a light coronary atherosclerosis (diameter stenosis <40%)(Group 1),11 patients with moderate coronary atherosclerosis (diameter stenosis 40%-80% in only one coronary artery) (Group 2), 11 patients with severe coronary atherosclerosis (diameter stenosis >80% in one or more coronary artery) (Group 3), 10 healthy subjects with normal coronary angiography (Group 5). Group 4 included 11 patients were on heparin. Circulating human lipoprotein lipase mRNA wAs detected by RT-PCR in all patients and healthy subjects. Results There was no statistic difference in any groups. There was no statistic difference between 21 patients with high serum triglyceride level and 13 patients with normal serum triglyceride level, between Group 3 and Group 4 (P>0.05). But the serum triglyceride level in Group 3 was higher than in Group 4 (P<0.05). Conclusion The similar expression of circulating human lipoprotein lipase mRNA is in healthy and patients with coronary atherosclerotic disease, who have different serum lipid level and severity of coronary stenosis. The expression of lipoprotein lipase mRNA is not stimulated by heparin.