Aim To investigate how Fructus Rosa Laevigata and Membrance of Chickens Gizzard influence plasma glucose and lipid levels and insulin levels in New Zealand white rabbits. Methods The rabbits were divided into 3 groups:normal group (4 rabbits) were fed common diet;control group (7 rabbits) were fed high fat and high sucrose; treatment group (7 rabbits), after 135 days fed high fat and high sucrose diets, Fructus Rosa Laevigata [50 g/(kg·d)] and Membrance of Chickens Gizzard [27 g/(kg·d)] were supplemented into the high fat and high sucrose diet;the rabbits were treated two courses and each treatment course was equal to 25 days. Results Before treatment, there was no difference among the 3 groups in glucose, triglyceride and insulin. After high fat and high sucrose diet was fed for 135 days, the glucose, triglyceride were increased (p<0.05), compared with normal group; after treatment, treated group was compared with the control group, glucose and triglyceride were decreased (p<0.05), and insulin level was no difference (p>0.05). Conclusion Fructus Rosa Laevigata and Membrance of Chickens Gizzard can decrease serum glucose and triglyceride levels.