Aim To study the relationship between angiotensin-converting enzyme (ACE)gene polymorphism and level of angiotensin Ⅱ (AngⅡ) in plasma with cerebral infarct (CI) in Chinese Han nationality. Methods 173 patients with cerebral infarct and 116 control subjects in Chinese Han were examined using polymorase chain reaction (PCR) and genetic methods. Plasma AngⅡ level determined by enzyme-linked immunosorbent assay (ELISA) method. Results ACE DD genotype appeared more common in patients of cerebral infarct compared with normal controls (0.39 versus 0.24, p<0.01), and it is important in frequency of ACE DD genotype abnormal increase in group of cerebral infarct onset age within 60 years. There were no significantly different in plasma AngⅡ level between cerebral infarct and control subjects, but cerebral infarct with ACE DD genotype group higher plasma AngⅡ level significantly higher than control group and cerebral infarct with ACE DI and II genotype (p<0.01), the level of plasma AngⅡ were increased most remarkably in group of cerebral infarct onset age within 60 years. Conclusions The ACE gene deletion polymorphism might serve as a risk factor for cerebral infarct in Chinese Han nationality while the level of plasma AngⅡ increased might related to group of cerebral infarct onset age within 60 years. Relate to gene deletion polymorphism.