Aim To investigate the effect of angiotensin Ⅱ (AngⅡ ) on the expression of monocyte chemoattractant protein-1 (MCP-1 ) and the activation of nuclear factor κB {NF-κB) in the endothelial cells under high glucose conditions, and the influence of losartan on MCP-1 and NF-KB. Methods MCP-1 mRNA was analyzed by reverse-transcriptase polymerase chain reaction (RT-PCR), and the level of MCP-1 in culture media was measured by means of sandwich ELISA method. NF-κB activation in endothelial cells was determined with laser scanning confocal microscope. Results Ang Ⅱ significantly increased the levels of MCP-1 mRNA and protein expression and NF-KB activation in the cultured endothelial cells. High glucose (25 mmol/L) could significantly increase the activation of NF-κB and the expression of MCP-1 in endothelial cells, while normal glu cose(5 mmol/L) could not. It was also shown that high glucose promoted AngⅡ -induced NF-KB activation and MCP-1 expression in endothelial cells. Losartan significantly inhibited MCP-1 mRNA and protein expression and markedly reduced the contents of MCP-1. Conclusion High glucose aggravate AngⅡ -induced NF-κB activation and MCP-1 expression in endothelial cell, which can be significantly inhibited by Losartan.