Aim To valuate the Association between heme oxygenase-1(HO-1)and angiographic morphology of coronary lesions in patients with coronary heart disease (CHD). Methods One hundred and thirty-six patients with coronary heart disease who underwent coronary angiography were selected in this study,and 40 patients with normal coronary artery were selected as control. According to angiographic morphology of coronary lesions,these patients were split into type I(smooth borders)group(n1=53),type Ⅱ(irregular borders)group(70)and type Ⅲ(long and irregular lesions)group(n3=13). The levels of HO-1 protein expression in monocyte and lymphocyte coming from patients with CHD were tested by immunohistochemistry and western blot. Computer picture analyzing system were also used to measure the levels of HO-1 protein expression. Results HO-1 protein is located in cell plasma; levels of HO-1 protein expression in patients with coronary heart disease were significantly higher than those of the controls(p<0.01); levels of HO-1 protein expression were the highest in type Ⅲ group,then type Ⅱ group,and at last the type I group(p<0.01). Conclusions There is a higher expression of HO-1 in patients with CHD,the levels of HO-1 protein are associated with angiographic morphology of coronary lesions,suspecting that HO-1 is associated with severity of coronary heart disease.