Aim To evaluate the relationship between G894T polymorphism(Glu298Asp) in the endothelial nitric oxide synthase(eNOS) gene and coronary heart disease(CHD) in population from various countries. Methods Odds ratios(OR) of genotype frequencies in CHD group against control group were analyzed.All the relevant studies were identified,those unqualified studies were excluded and the publication bias was evaluated.The Meta-analysis software(REVMAN4.2) was applied for investigating the heterogeneity among individual studies and for summarizing all the studies. Results Finally 12 studies entered the present study,including 5 891 cases and 3 392 controls.There was significant difference among individual studies(χ2=63.40,p<0.00001),so the random effect model was used to summarize all the studies.The pooled TT/(GT+GG) OR was 1.52,95% CI was 1.02～2.25(P=0.04). Conclusion The TT genotype of G894T polymorphism in eNOS gene is at moderately increased risk of CHD and may be one of the genetic risk factors of CHD.