Aim To observe the effects of monocyte chemotactic protein-1(MCP-1) and Fractalkine on tissue factor(TF) expression,proliferation and chemotaxis of vascular smoth muscle cell(VSMC). Methods The effects of MCP-1,Fractalkine and MCP-1+Fractalkine on TF antigen expression were analyzed by enzyme linked immunosorbent assay(ELISA).Their effects on VSMC proliferation were analyzed by MTT.And their effects on VSMC chemotaxis were evaluated by cell chemotaxis experiment. Results MCP-1 or Fractalkine could induce TF antigen in VSMC,but was less effective by MCP-1+Fractalkine than by MCP-1 or Fractalkine.MCP-1 and Fractalkine had competitive inhibition.Fractalkine had a strong impact on VSMC proliferation,while MCP-1+Fractalkine or MCP-1 had not.Fractalkine and MCP-1+Fractalkine induced the VSMC chemotaxis,while MCP-1 had little such function. Conclusions MCP-1 and Fractalkine had important effects on the atherosclerosis by working on TF expression,VSMC proliferation and chemotaxis.