Aim To study the role of a novel rabbits gene named Gnas during the hyperlipidemia.MethodsThe hyperlipidemia.rabbits were obtained after feeding atherogenic diet for 150 d.The differentially expressed gene was identified by fluorescent semi-quantitative PCR.The different nucleic acid expression level of Gnas in HepG2 cell line induced by cholesterol,25-hydryoxycholesterol,and human low density lipoprotein was analyzed by semi-quantitive RT-PCR.ResultsThe hyperlipidemia rabbits model has been successfully prepared.The total cholesterol levels in plasma and liver,triglyceride level in liver of hypercholesterolemia rabbits increased more than 10 folds,triglyceride level in plasma also significantly increased compared with those in control group.mRNA expression enhanced in the liver of hyperlipidemia rabbits.400ug/ml LDL induced Gnas expression increased 2.15 folds in HepG2 cell,though the same induced effects were not observed in cholesterol or 25-hydryoxycholesterol.ConclusionsThese results will contribute to reveal the molecular mechanism of the hyperlipidemia.