Aim To investigate the effect of angiotensin Ⅱ(AngⅡ) and its receptor antagonist on the matrix metalloproteinases 2(MMP-2) mRNA and protein expression in double stable AT2R expression cell lines of VSMC.Methods To establish the double stable cells of rat vascular smooth muscle cells(VSMC),in which the expression levels of AT2R is tightly controlled by doxycycline(Dox).The VSMC were treated with AngⅡ,Dox,CV-11974,PD123319.The effect of overexpression of AT2R on the mRNA and protein expression of MMP-2 in VSMC were detected and the effects of AT1R antagonist(CV-11974) and AT2R antagonist(PD123319) on aforementioned target were studied.Results The doxcycline(Dox) on gene expression system can generate high level and regulable expression of AT2R which tightly controled by addition/removal Dox.The expression of AT2R were induced obviously in double stable VSMC within 48 h by addition Dox and further,which enhanced after 72 h(p<0.01).In the well established double stable VSMC lines,addition of AngⅡ resulted in an increase of the expression of the MMP-2(p<0.01).The MMP-2 expression was decreased by treatment with Dox(p<0.01).Dox-induced decreased expression of MMP-2 were further enhanced by CV-11974(p<0.01),and were removed by PD123319.There are no difference in expression of MMP-2 between CV-11974,PD123319 simultaneously group with control group.Conclusion The enhancement effect of AngⅡ on the expression of MMP-2 is mediated by AT1R and inhibited by AT2R expression.It is suggestded that the regulable expression of AT2R can control the synthesis and degradation of Extracellular matrix effectively.