Aim To establish 2-dimensional gel electrophoresis images and seek differentially expressed plasma proteins of unstable angina pectoris patients with blood stasis syndrome.Methods 2-dimensional gel electrophoresis and Matrix-assisted laser desorption/ionization time of flight mass spectrometry were used to screen the differentially expressed plasma proteins among unstable angina pectoris with blood stasis syndrome patients and health volunteers.Results α1-acid glycoprotein,haptoglobin α1 chain,α1-antitrypsin,haptoglobin β chain and haptoglobin α2 chain were significantly highly expressed in the plasma of unstable angina pectoriswith blood stasis syndrome patients,while apolipoprotein AⅣ,apolipoprotein AⅠ and transthyretin were decreased in the plasma of unstable angina pectoris patients with blood stasis syndrome.Conclusions α1-acid glycoprotein,haptoglobin α1 chain,α1-antitrypsin,haptoglobin β chain,apolipoprotein AⅣ and apolipoprotein AⅠ are differentially expressed in the unstable angina pectoris patients with blood stasis syndrome and control group,and some differentially expressed proteins are correlated with inflammatory reaction or lipid metabolic disorder,and these proteins could provide clues for the study and discovery of new protein targets for antianginal drugs.