Aim To study the effect of ultra-filtration extract from the mixture of angelica sinensis and hedysarum polybotrys on the expressions of protein and mRNA of bcl-2 and bax gene in the apoptotic myocardial cells. Methods H2O2 of 400 μmol/L was used to build an oxidative stress-induced injury model in neonatal rat cardiomyocytes and then cardiomyocytes of each treatment group were treated with the Ultra-filtration extract(3.75,7.5,15 g/L).Morphological changes of cardiomyocytes were observed in microscope.The levels of bcl-2 and bax expression in cardiomyocytes were measured by RT-PCR and Western-blot. Results In cardiomyocytes of model group,the expression of bcl-2 and the rate of bcl-2/bax were decreased,and the expression of bax was increased significantly,compared with normal group(P<0.05).In cardiomyocytes of the high dosage group and the middle dosage group of the treatment,the bcl-2 expression was increased(P<0.05),the bax expression was decreased(P<0.05),and the rate of bcl-2/bax was increased(P<0.05),compared with model group.In cardiomyocytes of the little dosage group of the treatment,the expressions of bcl-2 and bax and the rate of bcl-2/bax were not changed significantly. Conclusion Ultra-filtration extract from the mixture of angelica sinensis and hedysarum polybotrys can effectively prevent and treat myocardial cells apoptosis,and its mechanism may be associated with up-regulation of the rate of bcl-2/bax.