Aim To explore the influence of advanced glycation end products(AGE) on inducing rat retinal microvascular endothelial cells(RMEC) to form new blood vessels by setting up a stable angiogenesis culture system on Matrigel and further discuss the effect of AGE on diabetic retinopathy. Methods RMEC were cultured in vitro and AGE-BSA were prepared.A stable angiogenesis culture system was set up on Matrigel.The experiment was divided into different concentration and different time groups.The expression of CD34 was detected by immunocytochemical method.The results were tested by calculating the number of tubules in microscopic observation and computer image-analysis.Finally the data was analyzed by statistics. Results RMEC were lumina formation on Matrigel.After treatment with AGE-BSA,the number of tubules increased and in certain ranges showing a dose-dependent and time-dependent manner(P<0.01). Conclusion AGE can promote inducing RMEC on Matrigel in vitro.