Aim To investigate the effect of resveratrol on the secretion of monocyte chemoattractant protein-1(MCP-1) in activated human umbilical vein endothelial cells(HUVEC),and the expression of CC chemokine receptor 2(CCR2) gene in human monocyte cell line THP-1 cells.Methods The expression level of CCR2 gene was measured by semiquantitative reverse expression polymerase-chain reaction in THP-1 cells.HUVEC were activated with tumor necrosis factor-α after preincubation of resveratrol.Then the secretion of MCP-1 in the cultivate supernatant of HUVEC was investigated by enzyme linked immunosorbent assay.Results The expression of CCR2 gene was inhibited by 10 μmol/L and 50 μmol/L resveratrol in monocyte (0.19±0.02 and 0.06±0.02 vs 0.73±0.15,P<0.01).The inhibition was increased with the dosage of resveratrol(1 μmol/L,10 μmol/L and 50 μmol/L,P<0.01).The secretion of MCP-1 protein in activated HUVEC was suppressed by 10 μmol/L and 50 μmol/L resveratrol (9 663.33±927.38 ng/L and 2 822.17±472.47 ng/L vs 16 595.67±1 667.39 ng/L,P<0.01).The suppression was enhanced with the increased dosage of resveratrol(P<0.01).Conclusion Resveratrol can inhibit the expression of CCR2 gene in THP-1 cell and suppress the secretion of MCP-1 in activated HUVEC.Both effects are in a dose-dependent manner,which may actively suppress chemotaxis of mononuclear cells.It may contribute to resveratrol's anti-atherosclerosis effects.