AimTo investigate the contribution of platelet activation and the damage marker of blood vessel endothelium in stroke.Methods300 SD rats were divided into three groups: model group, sham operated group and normal control group, the sham operated group were divided into sham operated group and sham operated+endothelium damage group; the model group were divided into hypertension group, endothelium damage group and hypertension+endothelium damage group.In the 12th week the rats were divided into artificial cold exposure (ACE) and non-ACE, only ACE were endured by artificial climate cabinet, blood samples were collected for measuring CD62p expression and brain tissues were got to analyse intercellular adhesion molecule-1 (ICAM-1) expression.ResultsIn model group, the CD62p and ICAM-1 expression were higher than other groups (P<0.05); the CD62p and ICAM-1 expression in ACE (model group) were higher than non-ACE group (P<0.05); the CD62p and ICAM-1 expression in hypertension+endothelium damage group was the highest in model group (P<0.05).ConclusionPersistent hypertension could damage the endothelium system of rat, artificial cold would worse this damage, and make it close to the state of pro-stroke.