Aim To explore the effects of lisinopril on the activities of Na+,K+-ATPase and Ca²⁺+-ATPase and mRNA expression levels of Na+,K+-ATPase α1-subunit and plasma membrane Ca²⁺+-ATPase isoform 1(PMCA1) in cultured thoracic aorta vascular smooth muscle cells (ASMC) isolated from spontaneously hypertensive rats (SHR). Methods ASMC were divided into four groups:Wistar-Kyoto (WKY) control,SHR control,Lisinopril(1×10-6)intervened SHR group and lisinopril(1×10-5)intervened SHR group. The activities of ion pumps were detected by spectrophotography and mRNA expression were measured by real time PCR. The content of angiotensin Ⅱ(AngⅡ) in cells-cultured medium were detected by radioimmunoassay. Results The activities of Na+,K+-ATPase,Ca²⁺+-ATPase and the mRNA expression levels of Na+,K+-ATPase α1-subunit and PMCA1 in ASMC from SHR were significantly lower than those from WKY control (P><0.01). Lisinopril significantly increased the activities of Na+,K+-ATPase and Ca²⁺+-ATPase and mRNA expression levels of Na+,K+-ATPase α1-subunit and PMCA1 in ASMC from SHR (P><0.01). AngⅡcontent of culture medium in ASMC from SHR was significantly more than those from WKY control(P><0.05),lisinopril attenuated AngⅡcontent of ASMC culture medium from SHR (P><0.05). Conclusion The decreased activities of Na+,K+-ATPase and Ca²⁺+-ATPase may be related to their lower expression of the mRNA in ASMC from SHR. The lisinopril may increase the activities of two ion pumps and upregrulae the mRNA expression of Na+,K+-ATPase α1-subunit and PMCA1 in ASMC from SHR through blocking the generation of AngⅡ.