Aim To investigate the effect of Tongfengkangding granule(TG) on anti-gout and the acute toxicity effect.Methods Sixty Kunming mouse were randomly divided into six groups(n=10): the normal goup,the hyperuricemia model group,high TG(12 g/kg),middle TG group(6 g/kg) and low TG group(3 g/kg),the allopurinol group(50 mg/kg).The treatment groups were orally administered once per day separately,the hyperuricemia model mouse was induced by injection of the pre-substance of hypoxanthine intraperitoneally.Serum uric acid level was determined,the effect of TG in rats with adjuvant arthritis induced by sodium urate,analgesic effect of TG in mouse with writhing test,and the acute toxicity effect of TG were investigated.Results The serum uric acid level was obviously decreased in hyperuricemia mouse treated with 12 g/kg TG(P<0.05),which could inhibit acute inflammation in model animals and had significant effect on analgesia,and obvious toxic reaction was not observed and the maximum dose was 121.50 g/kg for mice in acute toxicity experiment.Conclusion TG had a remarkable effect on hyperuricemia and anti-gout,and oral application had a high security.