AimTo observe the effect of Amlodipine on the expression of platelet-derived growth factor-B(PDGF-B) and nuclear factor-κB (NF-κB) in human umbilical vein endothelial cell (HUVEC-12) induced by oxidized low density lipoprotein (ox-LDL), further to explore the related mechanism of Amlodipine through its role on ox-LDL/NF-κB/PDGF-B.MethodsAfter pretreated with different concentration (0, 0.1, 1.0, 10.0 μmol/L) of Amlodipine for 0.5 h and treated with 50 mg/L ox-LDL for 24 h, the expressions of PDGF-B and NF-κB protein were respectively detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western Blot.Then, Pyrrolidinedithiocarbamic acid, ammonium salt(PDTC), a kind of NF-κB inhibitor was added, and RT-PCR and Western Blot were used to detect the expression of PDGF-B and NF-κB.ResultsWith the increasing concentration of Amlodipine, the PDGF-B and NF-κB protein expressions were all down-regulated obviously.PDTC not only down-regulated the NF-κB protein expression, but also the PDGF-B expression, as effectively as 10.0 μmol/L Amlodipin group.ConclusionAmlodipine can down-regulate the PDGF-B expression in HUVEC-12 induced by ox-LDL through NF-κB.