AimTo investigate the effect of mitofusin2 (Mfn2) with the removal of protein kinase A (PKA) phosphorylation site (Mfn2-PKA(△)) on promoting the apoptosis of vascular smooth muscle cell (VSMC) and related signaling pathway.MethodsVSMC were transfected with adenovirus vector encoding Mfn2-PKA(△) or Mfn2 (Adv-Mfn2-PKA(△), Adv-Mfn2).The abundance of Mfn2-PKA(△) protein and Mfn2 protein were determined by Western Blot analysis using Mfn2 phosphorylated antibody.Laser confocal microscopy(LCMS) was used to observe the location of proteins.The effect of Adv-Mfn2-PKA(△) on the apoptosis of VSMC was explored by cell death ELISA.Western Blot were used to detect the expression of p-Akt.ResultsBoth Mfn2-PKA(△) and Mfn2 were located at the outmembrane of mitochondria.ELISA assay showed Mfn2-PKA(△) had no effect on promoting the apoptosis of VSMC compared with Mfn2 (p<0.01).Western Blot indicated that overexpression of Mfn2-PKA(△) gene could not down-regulate the phosphorylation of Akt (p<0.01).ConclusionMfn2-PKA(△) could not induce the apoptosis of VSMC.PKA phosphorylation site plays an important role in regulating the function of Mfn2 gene.