Aim To investigate the effects of ouabain or atrial natriuretic peptide （ANP） on angiotensinⅡ secreted by artery smooth muscle cells （ASMC） in WKY rats and spontaneously hypertensive rats （SHR）. Methods The thoracic ASMC isolated from SHR and WKY rats were cultured and interfered with different concentrations of ANP or ouabain （1×10^－9 mol/L, 1×10^－8 mol/L and 1×10^－7 mol/L） respectively. The levels of angiotensinⅡ in culture-medium were measured by radioimmunoassay before and after 3, 6, 12, and 24 hours of using ouabain or ANP （1×10^－7 mol/L）. Results AngiotensinⅡ level in WKY rats was lower than that in SHR. After using ouabain, angiotensinⅡ level of two kinds of rats was remarkably increased, but in WKY rats angiotensinⅡ level reached the peak after 12 hours, while in SHR reached the peak after 6 hours. With the increasing concentration of ouabain, angiotensinⅡ level in WKY rats was increased in a concentration-dependent manner, and in SHR increased in a concentration-independent manner. After using ANP, the level of angiotensinⅡ of two kinds of rats was significantly increased, but in WKY rats reached the peak after 3 hours, and in SHR increased in a time-dependent manner. With the increase of concentration of ANP, the level of angiotensinⅡ went down in WKY rats, whereas went up in SHR. Conclusions Ouabain or ANP can promote the secretion of angiotensinⅡ of ASMC in both WKY rats and SHR, and the secretion peak of angiotensinⅡ induced by ouabain is in advance in SHR. The secretion of AngiotensinⅡ induced by ANP is significantly abnormal in SHR.