Aim Mitofusion 2 （Mfn2） is a gene which can negatively regulate the hyperplasia of vascular smooth muscle cells （VSMC）. The gene is down-regulated in the VSMC of essential hypertensive patients. However,the potential mechanism is undiscovered. The object of this study is to investigate the mechanism of Mfn2 gene transcription and regulation by bioinformatics. Method By using online software,the promoter distribution,transcription factors and their binding sites,CpG island,TATA box,and conservative region were analyzed in the 5′ uncoding region of Mfn2 gene. Simultaneously,the distribution and function change of SNP were studied. Results There were five promoters in the 5′ uncoding region of Mfn2 gene,which two of them played a key role in the transcription of Mfn2. Sixty nine transcription factors′ binding sites were found in the two conservative districts. These transcription factors were mainly associated with inflammation,oxidative stress,hyperplasia and differentiation of cells,MAPK and STAT signal pathway. One CpG island was found. Eight of 15 TATA boxes were found in the region of －2000 bp upstream of the transcription origin site. Thirty two SNP sites were found,nine were in the core promoter region,and five may affect the transcription of Mfn2. Conclusion The core promoter distributes in the －400 bp region of 5′ uncoding region of Mfn2. There were a lot of transcription factors which were closely related with the function of Mfn2 existed. In addition,some SNPs which may affect the transcription regulation of Mfn2 were found in the promoter region.