Aim To investigate the effects of insulin-like growth factor binding protein-4 （IGFBP4） on vascular endothelial cell senescence.Methods Human umbilical vein endothelial cells （HUVEC） were cultured in vitro. A HUVEC aging model was established through cell passaging. Lentivirus expressing IGFBP4 or si-IGFBP4,and empty vector transfected HUVEC,then the effects of IGFBP4 on the aging process of these endothelial cells were studied by using light microscopy,senescence-associated （SA） β-galactosidase staining,and cell cycle analysis.Results After lentivirus transfection,the HUVEC passage number of the overexpressing IGFBP4（+） group （IGFBP（+） group） was higher than that of the empty vector group （control group） and IGFBP4 silenced groups （IGFBP4（－） group） （14.33±0.67 vs 9.67±0.33 and 10.67±0.33,P<0.05） The cell senescence rate of the IGFBP4（+） group was lower than that of the control group and IGFBP4（－） group in every passage （take the 6th passage for example,24.60%±0.90% vs 34.92%±0.92% and 33.74%±0.62%,P<0.05） Along with degenerate,the decreased cell proliferation index （△PI） from young passage （2nd passage） to old passage （6th passage） of IGFBP4（+） was smaller than control group and IGFBP4（－） group （10.35%±0.57% vs 14.90%±0.85% and 13.99%±0.65%,P<0.05）.Conclusions IGFBP4 overexpression could inhibit HUVEC senescence.