趋化因子CX3C受体1基因rs3732378多态性与急性冠状动脉综合征的相关性
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(1.锦州医科大学研究生学院,辽宁省锦州市 121000;2.沈阳军区总医院心血管内科,辽宁省沈阳市 110000)

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童方念,硕士,主要从事冠心病的治疗与基础研究,E-mail为526644768@qq.com。

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辽宁省科学技术计划面上项目(2015020400)


Correlation between rs3732378 polymorphism of chemokine CX3C receptor 1 gene and acute coronary syndrome
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1.Graduate School of Jinzhou Medical University, Jinzhou, Liaoning 121000, China;2.Department of Cardiology, General Hospital of Shenyang Military Region, Shenyang, Liaoning 110000, China)

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    摘要:

    目的 探讨趋化因子CX3C受体1(CX3CR1)基因rs3732378单核苷酸多态性与急性冠状动脉综合征(ACS)的相关性。 方法 连续收集中国北方汉族人群951例,其中男性520例,女性431例,年龄35~75岁。根据冠状动脉造影(CAG)结果分为2组:(1)病例组(n=512):ACS患者;(2)对照组(n=439):非冠心病患者。病例组根据CAG检查血管病变支数分为3个亚组。采用测序法测定CX3CR1基因rs3732378单核苷酸多态位点的基因型。用多因素Logistic回归分析CX3CR1基因rs3732378多态性与ACS发病风险的关系。应用酶联免疫吸附法检测血浆中趋化因子CX3C配体1(CX3CL1)表达水平。 结果 两组CX3CR1基因rs3732378的基因型及等位基因的分布频率无显著性差异(P>0.05)。rs3732378多态位点与ACS发病风险的总体和分层分析结果表明,CX3CR1基因rs3732378多态位点的3种基因型TT、TC和CC均不能增加ACS的发病风险(P>0.05)。亚组分析显示,rs3732378多态位点的基因型和等位基因与冠状动脉血管病变支数无相关性(χ2=0.135,P=0.998;χ2=0.026,P=0.987)。病例组和对照组血浆中CX3CL1表达水平在rs3732378三种基因型无差异(P>0.05)。 结论 CX3CR1基因rs3732378多态位点不是ACS的易感基因,rs3732378多态性没有增加中国北方汉族人群ACS的风险。

    Abstract:

    Aim To investigate the correlation between rs3732378 single nucleotide polymorphism of chemokine CX3C receptor 1 (CX3CR1) gene and acute coronary syndrome (ACS). Methods 951 cases of Han population in northern China were collected continuously, of which 520 cases were male, 431 cases were female, and the age was 35-75 years old. According to the results of coronary angiography (CAG), the selected subjects were divided into two groups:(1)case group (n=512):ACS patients; (2)control group (n=439):non coronary heart disease patients. The case group was divided into three subgroups according to the number of vascular lesions that were examined by CAG. The genotypes of rs3732378 single nucleotide polymorphisms of CX3CR1 gene were determined by sequencing. Multiple factor Logistic regression was used to analyze the relationship between the CX3CR1 gene rs3732378 polymorphism and the risk of ACS. The expression of chemokine CX3C ligand 1 (CX3CL1) in plasma was detected by enzyme-linked immunosorbent assay. Results There was no significant difference in the distribution frequency of rs3732378 genotypes and alleles of CX3CR1 gene in the two groups (P>0.05). The overall and stratified analysis of rs3732378 polymorphisms and ACS risk showed that three genotypes TT, TC and CC of CX3CR1 rs3732378 polymorphisms did not increase the risk of ACS (P>0.05). Subgroup analysis showed that the genotype and allele of rs3732378 polymorphic loci were not related to the number of coronary artery lesion (χ2=0.135, P=0.998; χ2=0.026, P=0.987). There was no significant difference in the expression level of CX3CL1 among three rs3732378 genotypes in the case group and the control group (P>0.05). Conclusion The rs3732378 polymorphism of CX3CR1 gene is not a susceptible gene of ACS, and rs3732378 polymorphism does not increase the risk of ACS in the Han population of northern China.

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童方念,张效林,程茗慧,苏旭,韩雅玲.趋化因子CX3C受体1基因rs3732378多态性与急性冠状动脉综合征的相关性[J].中国动脉硬化杂志,2017,25(12):1247~1252.

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  • 收稿日期:2017-08-09
  • 最后修改日期:2017-09-22
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  • 在线发布日期: 2017-12-28