With the aging of population, the burden of medical is aggravated. As a new treatment method, stem cell transplantation has reversed aging and its accompanying dysfunction. Mesenchymal stem cell, as a type of stem cell with both regeneration and immunoregulatory functions, can promote the regeneration and repair of aging tissues through direct differentiation of cell replacement and cell empowerment. At the same time, recent studies have found that continuous centrifugation can separate exosomes which is the main therapeutic carrier from mesenchymal stem cell. Senescence-related miRNA in exosomes is helpful to elucidate the molecular mechanism of anti-aging treatment of mesenchymal stem cell.