Aim To investigate the relationship between serum apolipoprotein B (ApoB)/apolipoprotein A1 (ApoA1), creatine kinase isoenzyme (CK-MB) and cardiac troponin I (cTnI) levels and the degree of coronary artery stenosis in patients with coronary heart disease (CHD). Methods 120 patients with CHD were selected as the CHD group, and 40 healthy people who underwent a physical examination during the same period were selected as the normal group. The differences of ApoB/ApoA1, CK-MB and cTnI between normal group and CHD group were compared. In addition, patients in the CHD group were divided into three groups of mild, moderate, and severe coronary artery disease according to the Gensini scores, 25 cases, 52 cases, and 43 cases in each group, respectively. The differences of ApoB/ApoA1, CK-MB and cTnI in CHD patients with different stenosis degrees were compared. In addition, Spearman method was used to analyze the correlation between ApoB/ApoA1, CK-MB and cTnI levels and the degree of coronary artery stenosis. Results The levels of ApoB/ApoA1, CK-MB and cTnI in the CHD group were evidently higher than those in the normal group (P＜0.05). The levels of ApoB/ApoA1, CK-MB and cTnI in the moderate CHD group were evidently higher than those in the mild CHD group (P＜0.05), and the severe CHD group were evidently higher than those in the mild and moderate CHD groups (P＜0.05). ApoB/ApoA1, CK-MB and cTnI levels were positively correlated with the degree of coronary artery stenosis (r＝0.4,0.0,0.930, P＜0.05). Conclusion The levels of serum ApoB/ApoA1, CK-MB and cTnI in patients with CHD are positively correlated with the degree of coronary artery stenosis.