The highly dynamic tight junctions between the endoplasmic reticulum and mitochondria are known as mitochondria-associated ER membranes. These domains are essential for basic biological processes, including lipid metabolism, calcium homeostasis, mitochondrial dynamics, autophagy and apoptosis, endoplasmic reticulum stress and inflammation. Many studies have proved the abnormal amount, structure or function of mitochondria-associated ER membranes are related to the occurrence and development of cardiovascular diseases. This paper summarized the functions of mitochondria-associated ER membranes and its roles and possible mechanism in cardiovascular diseases, and provided theoretical references for mitochondria-associated ER membranes to become new targets for cardiovascular therapy.