反义c—myc寡聚核苷酸对动脉平滑肌细胞的生长抑制及其Myc蛋白合成的抑制
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An Oligomer Complementary To C-myc-encoded mRNA Inhibits Proliferation of Arterial Smooth Muscle Cells And Production of myc Protein
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    摘要:

    本文合成了反义和正义c—myc 5′—端编码区的前6个密码子。分别导入培养的WKY太鼠主动脉平滑肌细胞(SMCs),同时用内皮素(ET)诱导SMCs增殖,反义c—myc寡核苷酸(ODNs)抗细胞增殖作用随浓度(6μmol/L至12μmol/L)增加而增加。用12μmol/L反义ODNs抗细胞增殖能力随时间廷长而下降。免疫组化显示受抑制细胞Myc水平较同期对照组或正义ODNs组低。本研究为反义ODNs抑制外源性生长因子或细胞因子诱导靶基因的高表达和细胞增殖提供了新的线索,同时为探讨癌基因表达调控与动脉SMCs增殖的关系提供了一种新方法。

    Abstract:

    When antisense myc oligodeoxynucleotide (ODNs) (18-mer) were introduced into the medium of the smooth muscle cells (SMCs) of WKY in concentrations ranging from 6 to 12 uM,the 18-met ODNs,in a concentration-related manner,decreased SMCs growth induced by endothelin (10~(-7)mol/L). The inhibitory effect (12umol/L) was about 43% in 12h, 33% in 24h and 27% in 36h. Sense ODNs had little effect. Meanwhile, the anti c-myc had inhibitory effect on production of Myc protein of the SMCs. Our results demonstrate that an antisense ODNs directed at the messenger RNA of myc decreases the expression of myc gone product and reduces SMCs proliferation induced by endothelin.

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杨和平,刘革修,万腊香,涂玉林,杨永宗,董薇,李岱宗,周爱儒,汤健,黄曦.反义c—myc寡聚核苷酸对动脉平滑肌细胞的生长抑制及其Myc蛋白合成的抑制[J].中国动脉硬化杂志,1993,1(1):4~8.

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