内源性高甘油三酯血症患者淋巴细胞LDL受体及LDL结合活性的研究
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LDL Receptors of Lymphocytes and Binding of LDL by Non-parenchymal Cells of Rat Liver in Endogenous Hypertriglyceridemics
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    用酶联法对内源性高甘油三酯血症患者血淋巴细胞LDL受体进行了分析。结果表明,高甘油三酯血症患者淋巴细胞LDL受体结合容量Bmax为57.5±13.2ng/mg细胞蛋白,较血脂正常者的38.2±9.3ng/mg细胞蛋白显著升高(p<0.05),而其Kd值无改变(12.0±3.2对13.3±4.1μg/ml,p>0.05)。患者血浆LDL与大鼠肝非实质细胞的结合活性较正常对照组显著降低(p<0.05)。

    Abstract:

    A study on LDL receptors of lymphocytes and plasma LDL binding activity to liver non-parenchymal cells of rat in 5 normal persons and endogenous hypertriglyceridemlcs(whose plasma triglycerides levels were 0. 87±0. 32 and 3.07 ±1.67 mmol/L respectively) was made. Plasma LDL was isolated by density gradient ultracentrifugation. LDL receptors of lymphocytes were measured by a enzyme-linked method developed by this laboratory.125 I-labelled-LDL was prepared by IC1 method. Liver non-parenchymal cells(NPC) of rat were prepared by collagenase method.125ILDL binding to rat liver NPC was measured by Goldstein and Brown' s method. The results showed that the Bmax of LDL binding to lymphocytes from hypertriglyceridemic group was significantly higher than that of normal group(57.5±13.2:38.2±9.3 ng/mg cell protein,p<0. 05), but there was no difference in affinity(Kd) between the normal and hypertriglyceridemic groups(12.0±3.2:13.3±4.1μg/ml, p>0. 05).The binding activity of plasma LDL from hypertriglyceridemics to rat liver NPC was significantly lower than that of LDL from the normal group(p<0. 05). The results are disscused.

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刘秉文,洪瑛,傅明德.内源性高甘油三酯血症患者淋巴细胞LDL受体及LDL结合活性的研究[J].中国动脉硬化杂志,1994,2(1):6~8.

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