Aim In order to study the role of insulin-likegrowth of factor-1 receptor (IGFR) in the growth ofvascular smooth muscle cells(VSMC).Methods Antisense and mismatch of oligodeoxynu-cleotides (ODN) of IGFR were used to study smoothmuscle cell of mouse arteries.Results The incorporation of 3H-thymidine inVSMC handling by antisense of ODN decreased 64%compared with normal control group. The determi-nation of the receptor number in binding experimentdecreases 52% cell handing by as incubated differentconcentration of IGF-l (1 ~ 50 ng) caused a dose de-pendent increase in 3H-thymidine incorporation stimu-lated by 20 μg/L IGF-1 was reduced by 62% in anti-sense treated cells the effect of cells incubation of an-giotensin Ⅱ in antisense handling cells was not al-tered,the receptor quantities didn't reduce.Conclusion This experiment showed the impor-tance of ligand-receptor system to VSMC. The den-sity of receptor p1ays a key role to the growth ofVSMC the decreasing of IGF-1 receptor numbercaused by antisense was due to the decreasing of mR-NA in IGF-1 receptor. This hypothesis has beenproved in the experiment of nudease protection.