To study the possibility of expressionof human lecithin cholesterol acyltransferase (LCAT)in skeletal muscles. Constraction of a plasmid vectorcontaining the human LCAT cDNA: transfer the vec-tor into myogenic cell line C2, and detection of LCATactivity in the culture medium. A expression vector ofhuman LCAT drived by CMV E/P, PCMV-LCAT wasconstructed, which successfully expressed in C2 cells.The LCAT activity was detected in the culture medi-um. It seems possible to chance plasma LCAT level bytransfer of LCAT gene into skeletal muscle.