Aim To develop a new method for microdetermination of taurine the composition of antiatherosclerosis, in animal tissues With reverse-phase high performance liquid chromatography (RP-HPLC) Methods Animal tissues were soaked in water to extract taurine. The extraction was concentrated by vacuum freezing and drying. Then using the mixed liquid of Phenylisothiocyanate (PITC), triethylamine,water and ethanol (1: 1:1: 7) as derivative to precolumn derived taurine with PITC. Under the following conditions: 1 mL/min flow rate, 254 nm wavelength and column temperature at 45℃, 991 HPLC was used to separate, determine taurine. Qualitative and quantitative analysls of the content of taurine in sample animal (slug ) tissues was done by using the approaches of peak increase and standard curve respectivelv.Results Under the condition of selected chromatogram, the retention time of taurine was 5.06 min with distinct qualitative phenomenon of peak increasing. The result was reliable. The lowest detectable amount was 4. 48 ng with the concentration of taurine between 5. 58 X 1O3～ 1. 79 X 105μg/L. The linear regression equation between peak area of the chromatogram and the content of taurine was: S = 3. 34 X 10-4W - 1. 09 X 10-3, with r =0. 9998. The relative standard deviation of the sample liquid was 1. 5 % (n =7 ), the added recovery of the sample was P = 99.57 %.Conclusion This approach of soaking the taurine of animal tissues with water is simple and convonient.It decreases the contamination. The derivative reaction is complete; the derivative of taurine is well separated within the column. This approach is a new analytical method for detecting the trace taurine in animal tissues.